1mqk
From Proteopedia
(New page: 200px<br /> <applet load="1mqk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mqk, resolution 1.28Å" /> '''Crystal structure o...) |
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caption="1mqk, resolution 1.28Å" /> | caption="1mqk, resolution 1.28Å" /> | ||
'''Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2'''<br /> | '''Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MQK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1MQK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: membrane protein]] | [[Category: membrane protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:25:31 2008'' |
Revision as of 14:25, 15 February 2008
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Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2
Overview
The antibody Fv fragment 7E2 has previously been employed in the induced, crystallization of the integral membrane protein cytochrome c oxidase from, Paracoccus denitrificans. The 1.3 A X-ray structure of the uncomplexed, antibody fragment reveals conserved water networks on the surfaces of the, framework regions. A novel consensus motif for water coordination, XX(S/T), is found along the edges of the beta-sandwich, where a water, molecule forms hydrogen bonds to the carbonyl O atom of a residue at, position N and the OG hydroxyl groups of conserved serines or threonines, at position N + 2. Multiple conformations were found in the hydrophobic, core for residues IleL21, LeuL33 and the disulfide bridges. An internal, water molecule that is compatible with only one of the three packing, states of the V(L) core suggests local 'breathing' of the variable domain., TrpH47, a conserved key residue of the V(H)/V(L) interface, is crucially, involved in the formation of the antigen-binding site by adopting a novel, conformation that specifically stabilizes the non-canonical CDR-L3 loop., Finally, a comparison with 7E2-cytochrome c oxidase complexes demonstrates, that binding of this membrane-bound antigen proceeds without major, conformational changes of the 7E2 antibody fragment.
About this Structure
1MQK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
1.3 A X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization., Essen LO, Harrenga A, Ostermeier C, Michel H, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):677-87. Epub 2003, Mar 25. PMID:12657787
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