1n2k
From Proteopedia
(New page: 200px<br /> <applet load="1n2k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n2k, resolution 2.75Å" /> '''Crystal structure o...) |
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caption="1n2k, resolution 2.75Å" /> | caption="1n2k, resolution 2.75Å" /> | ||
'''Crystal structure of a covalent intermediate of endogenous human arylsulfatase A'''<br /> | '''Crystal structure of a covalent intermediate of endogenous human arylsulfatase A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1N2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NDG and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cerebroside-sulfatase Cerebroside-sulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.8 3.1.6.8] Full crystallographic information is available from [http:// | + | 1N2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NDG:'>NDG</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cerebroside-sulfatase Cerebroside-sulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.8 3.1.6.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: phosphate esters hydrolysis]] | [[Category: phosphate esters hydrolysis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:26:42 2008'' |
Revision as of 14:26, 15 February 2008
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Crystal structure of a covalent intermediate of endogenous human arylsulfatase A
Contents |
Overview
The structures of human arylsulfatase A crystals soaked in solutions, containing 4-methylumbelliferyl phosphate and O-phospho-DL-tyrosine have, been determined at 2.7- and 3.2-A resolution, respectively. The, formylglycine in position 69, a residue crucial for catalytic activity, was unambiguously identified in both structures as forming a covalent bond, to the phosphate moiety. A hydroxyl group is present at the Cbeta of, residue 69 and the formation of one out of two possible stereomeric forms, is strongly favoured. The structures confirm the importance of the, gem-diol intermediate in the arylsulfatase's catalytic mechanism. The, presence of an apparently stable covalent bond is consistent with the weak, phosphatase activity observed for human arylsulfatase A. The structures of, the complexes suggest that phosphate ions and phosphate esters inhibit, arylsulfatase in non-covalent and covalent modes, respectively. The metal, ion present in the active site of arylsulfatase A isolated from human, placenta is Ca(2+) and not Mg(2+) as was found in the structure of the, recombinant enzyme.
Disease
Known disease associated with this structure: Metachromatic leukodystrophy OMIM:[607574]
About this Structure
1N2K is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Cerebroside-sulfatase, with EC number 3.1.6.8 Full crystallographic information is available from OCA.
Reference
Crystal structure of a covalent intermediate of endogenous human arylsulfatase A., Chruszcz M, Laidler P, Monkiewicz M, Ortlund E, Lebioda L, Lewinski K, J Inorg Biochem. 2003 Aug 1;96(2-3):386-92. PMID:12888274
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