2jap
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(New page: 200px<br /> <applet load="2jap" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jap, resolution 2.10Å" /> '''CLAVULANIC ACID DEH...)
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Revision as of 13:55, 29 October 2007
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CLAVULANIC ACID DEHYDROGENASE: STRUCTURAL AND BIOCHEMICAL ANALYSIS OF THE FINAL STEP IN THE BIOSYNTHESIS OF THE BETA-LACTAMASE INHIBITOR CLAVULANIC ACID
Overview
The ultimate step in the biosynthesis of the medicinally important, beta-lactamase inhibitor clavulanic acid is catalyzed by clavulanic acid, dehydrogenase (CAD). CAD is responsible for the NAPDH-dependent reduction, of the unstable intermediate clavulanate-9-aldehyde to yield clavulanic, acid. Here, we report biochemical and structural studies on CAD., Biophysical analyses demonstrate that CAD exists as dimeric and tetrameric, species in solution. The reaction performed by CAD was shown to be, reversible, allowing the use of clavulanic acid for activity analyses. The, crystal structure of CAD was solved using single-wavelength anomalous, diffraction with a seleno-methionine derivative. The structure reveals, that the individual monomers comprise a single domain possessing the, Rossmann ... [(full description)]
About this Structure
2JAP is a [Single protein] structure of sequence from [Streptomyces clavuligerus] with NDP and J01 as [ligands]. Full crystallographic information is available from [OCA].
Reference
Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta-lactamase inhibitor clavulanic acid., MacKenzie AK, Kershaw NJ, Hernandez H, Robinson CV, Schofield CJ, Andersson I, Biochemistry. 2007 Feb 13;46(6):1523-33. Epub 2007 Jan 19. PMID:17279617
Page seeded by OCA on Mon Oct 29 16:00:05 2007
