1ocw
From Proteopedia
(New page: 200px<br /> <applet load="1ocw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ocw, resolution 2.00Å" /> '''FREE CONFORMATION A...) |
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caption="1ocw, resolution 2.00Å" /> | caption="1ocw, resolution 2.00Å" /> | ||
'''FREE CONFORMATION AB2 OF THE IGE SPE-7'''<br /> | '''FREE CONFORMATION AB2 OF THE IGE SPE-7'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OCW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http:// | + | 1OCW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: multispecificity]] | [[Category: multispecificity]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:33:48 2008'' |
Revision as of 14:33, 15 February 2008
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FREE CONFORMATION AB2 OF THE IGE SPE-7
Overview
A single antibody was shown to adopt different binding-site conformations, and thereby bind unrelated antigens. Analysis by both x-ray, crystallography and pre-steady-state kinetics revealed an equilibrium, between different preexisting isomers, one of which possessed a, promiscuous, low-affinity binding site for aromatic ligands, including the, immunizing hapten. A subsequent induced-fit isomerization led to, high-affinity complexes with a deep and narrow binding site. A protein, antigen identified by repertoire selection made use of an unrelated, antibody isomer with a wide, shallow binding site. Conformational, diversity, whereby one sequence adopts multiple structures and multiple, functions, can increase the effective size of the antibody repertoire but, may also lead to autoimmunity and allergy.
About this Structure
1OCW is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.
Reference
Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:12610298
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