1op8
From Proteopedia
(New page: 200px<br /> <applet load="1op8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1op8, resolution 2.5Å" /> '''Crystal Structure of...) |
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caption="1op8, resolution 2.5Å" /> | caption="1op8, resolution 2.5Å" /> | ||
'''Crystal Structure of Human Granzyme A'''<br /> | '''Crystal Structure of Human Granzyme A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Granzyme_A Granzyme A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.78 3.4.21.78] Full crystallographic information is available from [http:// | + | 1OP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Granzyme_A Granzyme A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.78 3.4.21.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OP8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:34:50 2008'' |
Revision as of 14:34, 15 February 2008
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Crystal Structure of Human Granzyme A
Overview
Granzyme A (GzmA) belongs to a family of trypsin-like serine proteases, localized in cytoplasmic granules of activated lymphocytes and natural, killer (NK) cells. In contrast to the related granzyme B (GzmB), GzmA, forms a stable disulfide-linked homodimer and triggers target-cell death, in a caspase-independent way. Limited proteolysis of a high-molecular-mass, complex containing SET (also named putative HLA-associated protein II or, PHAPII), PHAPI (pp32, leucine-rich acidic nuclear protein) and HMG2 by, GzmA liberates NM23-H1, a Mg2+-dependent DNase that causes single-stranded, breaks in nuclear DNA. By analyzing the dimeric GzmA structure at a, resolution of 2.5 A, we determined the substrate-binding constraints and, selective advantages of the two domains arranged as a unique functional, tandem. The active sites of the two subunits point in opposite directions, and the nearby noncatalytic surfaces can function as exosites, presenting, substrates to the active site region of the adjacent partner in a manner, analogous to staphylokinase or streptokinase, which present plasminogen to, the cofactor-plasmin and cofactor-plasminogen complexes.
About this Structure
1OP8 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Granzyme A, with EC number 3.4.21.78 Full crystallographic information is available from OCA.
Reference
Crystal structure of the apoptosis-inducing human granzyme A dimer., Hink-Schauer C, Estebanez-Perpina E, Kurschus FC, Bode W, Jenne DE, Nat Struct Biol. 2003 Jul;10(7):535-40. PMID:12819770
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