1orf
From Proteopedia
(New page: 200px<br /> <applet load="1orf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1orf, resolution 2.40Å" /> '''The Oligomeric Stru...) |
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| - | [[Image:1orf. | + | [[Image:1orf.jpg|left|200px]]<br /><applet load="1orf" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1orf, resolution 2.40Å" /> | caption="1orf, resolution 2.40Å" /> | ||
'''The Oligomeric Structure of Human Granzyme A Reveals the Molecular Determinants of Substrate Specificity'''<br /> | '''The Oligomeric Structure of Human Granzyme A Reveals the Molecular Determinants of Substrate Specificity'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ORF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Granzyme_A Granzyme A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.78 3.4.21.78] Full crystallographic information is available from [http:// | + | 1ORF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Granzyme_A Granzyme A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.78 3.4.21.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:35:20 2008'' |
Revision as of 14:35, 15 February 2008
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The Oligomeric Structure of Human Granzyme A Reveals the Molecular Determinants of Substrate Specificity
Overview
The cell death-inducing serine protease granzyme A (GzmA) has a unique, disulfide-linked quaternary structure. The structure of human GzmA bound, to a tripeptide CMK inhibitor, determined at a resolution of 2.4 A, reveals that the oligomeric state contributes to substrate selection by, limiting access to the active site for potential macromolecular substrates, and inhibitors. Unlike other serine proteases, tetrapeptide substrate, preferences do not correlate well with natural substrate cleavage, sequences. This suggests that the context of the cleavage sequence within, a macromolecular substrate imposes another level of selection not observed, with the peptide substrates. Modeling of inhibitors bound to the GzmA, active site shows that the dimer also contributes to substrate specificity, in a unique manner by extending the active-site cleft. The crystal, structure, along with substrate library profiling and mutagenesis, has, allowed us to identify and rationally manipulate key components involved, in GzmA substrate specificity.
About this Structure
1ORF is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Granzyme A, with EC number 3.4.21.78 Full crystallographic information is available from OCA.
Reference
The oligomeric structure of human granzyme A is a determinant of its extended substrate specificity., Bell JK, Goetz DH, Mahrus S, Harris JL, Fletterick RJ, Craik CS, Nat Struct Biol. 2003 Jul;10(7):527-34. PMID:12819769
Page seeded by OCA on Fri Feb 15 16:35:20 2008
Categories: Granzyme A | Homo sapiens | Single protein | Bell, J.K. | Craik, C.S. | Fletterick, R.J. | Goetz, D.H. | Harris, J.L. | Mahrus, S. | SO4 | Hydrolase
