1ow3
From Proteopedia
(New page: 200px<br /> <applet load="1ow3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ow3, resolution 1.80Å" /> '''Crystal Structure o...) |
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| - | [[Image:1ow3. | + | [[Image:1ow3.jpg|left|200px]]<br /><applet load="1ow3" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1ow3, resolution 1.80Å" /> | caption="1ow3, resolution 1.80Å" /> | ||
'''Crystal Structure of RhoA.GDP.MgF3-in Complex with RhoGAP'''<br /> | '''Crystal Structure of RhoA.GDP.MgF3-in Complex with RhoGAP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OW3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, GDP and MGF as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1OW3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GDP:'>GDP</scene> and <scene name='pdbligand=MGF:'>MGF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transition state]] | [[Category: transition state]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:36:35 2008'' |
Revision as of 14:36, 15 February 2008
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Crystal Structure of RhoA.GDP.MgF3-in Complex with RhoGAP
Overview
The formation of complexes between small G proteins and certain of their, effectors can be facilitated by aluminum fluorides. Solution studies, suggest that magnesium may be able to replace aluminum in such complexes., We have determined the crystal structure of RhoA.GDP bound to RhoGAP in, the presence of Mg(2+) and F(-) but without Al(3+). The metallofluoride, adopts a trigonal planar arrangement instead of the square planar, structure of AlF(4)(-). We have confirmed that these crystals contain, magnesium and not aluminum by proton-induced X-ray emission spectroscopy., The structure adopted by GDP.MgF(-) possesses the stereochemistry and, approximate charge expected for the transition state. We suggest that, MgF3(-) may be the reagent of choice for studying phosphoryl transfer, reactions.
About this Structure
1OW3 is a Protein complex structure of sequences from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
MgF(3)(-) as a transition state analog of phosphoryl transfer., Graham DL, Lowe PN, Grime GW, Marsh M, Rittinger K, Smerdon SJ, Gamblin SJ, Eccleston JF, Chem Biol. 2002 Mar;9(3):375-81. PMID:11927263
Page seeded by OCA on Fri Feb 15 16:36:35 2008
Categories: Homo sapiens | Protein complex | Eccleston, J.F. | Gamblin, S.J. | Graham, D.L. | Grime, G.W. | Lowe, P.N. | Marsh, M. | Rittinger, K. | Smerdon, S.J. | GDP | MG | MGF | Complex | Gap | Gtpase | Transition state
