1p2s
From Proteopedia
(New page: 200px<br /> <applet load="1p2s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p2s, resolution 2.45Å" /> '''H-Ras 166 in 50% 2,...) |
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| - | [[Image:1p2s. | + | [[Image:1p2s.jpg|left|200px]]<br /><applet load="1p2s" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1p2s" size=" | + | |
caption="1p2s, resolution 2.45Å" /> | caption="1p2s, resolution 2.45Å" /> | ||
'''H-Ras 166 in 50% 2,2,2 triflouroethanol'''<br /> | '''H-Ras 166 in 50% 2,2,2 triflouroethanol'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P2S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, GNP and ETF as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1P2S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GNP:'>GNP</scene> and <scene name='pdbligand=ETF:'>ETF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P2S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:38:27 2008'' |
Revision as of 14:38, 15 February 2008
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H-Ras 166 in 50% 2,2,2 triflouroethanol
Contents |
Overview
Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP, were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50%, isopropanol. The disordered switch II region of Ras is ordered in the, presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone, conformation of switch II in these organic solvents is the same as in the, Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a, biologically relevant form. Key polar interactions that stabilize the, ordered switch are enhanced in the presence of hydrophobic cosolvents., These results suggest that hydrophobic solvents can be used in general to, order short biologically relevant segments of disordered regions in, protein crystals by favoring H-bonding interactions between atoms that are, highly solvated and mobile in aqueous solution.
Disease
Known diseases associated with this structure: Bladder cancer, somatic OMIM:[190020], Costello syndrome OMIM:[190020], Thyroid carcinoma, follicular, somatic OMIM:[190020]
About this Structure
1P2S is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Organic solvents order the dynamic switch II in Ras crystals., Buhrman G, de Serrano V, Mattos C, Structure. 2003 Jul;11(7):747-51. PMID:12842038
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