1p9f
From Proteopedia
(New page: 200px<br /> <applet load="1p9f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p9f" /> '''NMR Structure of Neurokinin B from DYANA'''...) |
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'''NMR Structure of Neurokinin B from DYANA'''<br /> | '''NMR Structure of Neurokinin B from DYANA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P9F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1P9F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structures from dyana]] | [[Category: structures from dyana]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:39:26 2008'' |
Revision as of 14:39, 15 February 2008
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NMR Structure of Neurokinin B from DYANA
Overview
Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety, of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain, insight into structure-function relationship, three-dimensional structure, of NKB has been investigated using CD spectropolarimetry and, two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy, in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of, resonances have been made with the aid of correlation spectroscopy, (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect, Spectroscopy (NOESY) experiments. Distance constraints obtained from the, NMR data have been used to generate a family of structures, which have, been refined using restrained energy minimization and dynamics. Our data, show that a helical structure is induced in NKB, in presence of, perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model, system. Further, the conformation adopted by NKB in presence of DPC, micelles represents a structural motif typical of neurokinin-3 selective, agonists.
About this Structure
1P9F is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Three dimensional structure of mammalian tachykinin peptide neurokinin B bound to lipid micelles., Mantha AK, Chandrashekar IR, Baquer NZ, Cowsik SM, J Biomol Struct Dyn. 2004 Oct;22(2):137-48. PMID:15317475
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