1q0x
From Proteopedia
(New page: 200px<br /> <applet load="1q0x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q0x, resolution 1.60Å" /> '''Anti-morphine Antib...) |
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| - | [[Image:1q0x. | + | [[Image:1q0x.jpg|left|200px]]<br /><applet load="1q0x" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1q0x, resolution 1.60Å" /> | caption="1q0x, resolution 1.60Å" /> | ||
'''Anti-morphine Antibody 9B1 Unliganded Form'''<br /> | '''Anti-morphine Antibody 9B1 Unliganded Form'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 and PG4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1Q0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PG4:'>PG4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: fab fragment]] | [[Category: fab fragment]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:42:12 2008'' |
Revision as of 14:42, 15 February 2008
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Anti-morphine Antibody 9B1 Unliganded Form
Overview
The crystal structures of an anti-morphine antibody 9B1 (to 1.6A, resolution) and its complex with morphine (to 2.0 A resolution) are, reported. The morphine-binding site is described as a shallow depression, on the protein surface, an unusual topology for a high-affinity ( Ka, approximately 10(9) M(-1)) antibody against a small antigen. The polar, part of the ligand is exposed to solvent, and the cationic nitrogen atom, of the morphine molecule is anchored at the bottom of the binding site by, a salt-bridge to a glutamate side-chain. Additional affinity is provided, by a double cation-pi interaction with two tryptophan residues. Comparison, of the morphine complex with the structure of the free Fab shows that a, domain closure occurs upon binding of the ligand.
About this Structure
1Q0X is a Single protein structure of sequence from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine., Pozharski E, Wilson MA, Hewagama A, Shanafelt AB, Petsko G, Ringe D, J Mol Biol. 2004 Mar 26;337(3):691-7. PMID:15019787
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