1qcy
From Proteopedia
(New page: 200px<br /> <applet load="1qcy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qcy, resolution 2.30Å" /> '''THE CRYSTAL STRUCTU...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1qcy. | + | [[Image:1qcy.jpg|left|200px]]<br /><applet load="1qcy" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1qcy" size=" | + | |
caption="1qcy, resolution 2.30Å" /> | caption="1qcy, resolution 2.30Å" /> | ||
'''THE CRYSTAL STRUCTURE OF THE I-DOMAIN OF HUMAN INTEGRIN ALPHA1BETA1'''<br /> | '''THE CRYSTAL STRUCTURE OF THE I-DOMAIN OF HUMAN INTEGRIN ALPHA1BETA1'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1QCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCY OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 23: | ||
[[Category: rossman fold]] | [[Category: rossman fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:44:07 2008'' |
Revision as of 14:44, 15 February 2008
|
THE CRYSTAL STRUCTURE OF THE I-DOMAIN OF HUMAN INTEGRIN ALPHA1BETA1
Overview
Integrin alpha(1)beta(1) is one of four collagen-binding integrins in, humans. Collagens bind to the alphaI domain and in the case of alpha(2)I, collagen binding is competitively inhibited by peptides containing the, RKKH sequence and derived from the metalloproteinase jararhagin of snake, venom from Bothrops jararaca. In alpha(2)I, these peptides bind near the, metal ion-dependent adhesion site (MIDAS), where a collagen (I)-like, peptide is known to bind; magnesium is required for binding. Published, structures of the ligand-bound "open" conformation of alpha(2)I differs, significantly from the "closed" conformation seen in the structure of, apo-alpha(2)I near MIDAS. Here we show that two peptides, CTRKKHDC and, CARKKHDC, derived from jararhagin also bind to alpha(1)I and competitively, inhibit collagen I binding. Furthermore, calorimetric and fluorimetric, measurements show that the structure of the complex of alpha(1)I with, Mg(2+) and CTRKKHDC differs from structure in the absence of peptide. A, comparison of the x-ray structure of apo-alpha(1)I ("closed" conformation), and a model structure of the alpha(1)I ("open" conformation) based on the, closely related structure of alpha(2)I reveals that the binding site is, partially blocked to ligands by Glu(255) and Tyr(285) in the "closed", structure, whereas in the "open" structure helix C is unwound and these, residues are shifted, and the "RKKH" peptides fit well when docked. The, "open" conformation of alpha(2)I resulting from binding a collagen, (I)-like peptide leads to exposure of hydrophobic surface, also seen in, the model of alpha(1)I and shown experimentally for alpha(1)I using a, fluorescent hydrophobic probe.
About this Structure
1QCY is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Jararhagin-derived RKKH peptides induce structural changes in alpha1I domain of human integrin alpha1beta1., Nymalm Y, Puranen JS, Nyholm TK, Kapyla J, Kidron H, Pentikainen OT, Airenne TT, Heino J, Slotte JP, Johnson MS, Salminen TA, J Biol Chem. 2004 Feb 27;279(9):7962-70. Epub 2003 Dec 4. PMID:14660600
Page seeded by OCA on Fri Feb 15 16:44:07 2008
