1r74
From Proteopedia
(New page: 200px<br /> <applet load="1r74" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r74, resolution 2.55Å" /> '''Crystal Structure o...) |
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caption="1r74, resolution 2.55Å" /> | caption="1r74, resolution 2.55Å" /> | ||
'''Crystal Structure of Human Glycine N-Methyltransferase'''<br /> | '''Crystal Structure of Human Glycine N-Methyltransferase'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R74 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BME and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycine_N-methyltransferase Glycine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.20 2.1.1.20] Full crystallographic information is available from [http:// | + | 1R74 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=BME:'>BME</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycine_N-methyltransferase Glycine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.20 2.1.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R74 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: human]] | [[Category: human]] | ||
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Revision as of 14:47, 15 February 2008
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Crystal Structure of Human Glycine N-Methyltransferase
Contents |
Overview
Glycine N-methyltransferases (GNMTs) from three mammalian sources were, compared with respect to their crystal structures and kinetic parameters., The crystal structure for the rat enzyme was published previously. Human, and mouse GNMT were expressed in Escherichia coli in order to determine, their crystal structures. Mouse GNMT was crystallized in two crystal, forms, a monoclinic form and a tetragonal form. Comparison of the three, structures reveals subtle differences, which may relate to the different, kinetic properties of the enzymes.The flexible character of several loops, surrounding the active site, along with an analysis of the active site, boundaries, indicates that the observed conformations of human and mouse, GNMTs are more open than that of the rat enzyme. There is an increase in, kcat when going from rat to mouse to human, suggesting a correlation with, the increased flexibility of some structural elements of the respective, enzymes.
Disease
Known disease associated with this structure: Glycine N-methyltransferase deficiency OMIM:[606628]
About this Structure
1R74 is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Glycine N-methyltransferase, with EC number 2.1.1.20 Full crystallographic information is available from OCA.
Reference
Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes., Pakhomova S, Luka Z, Grohmann S, Wagner C, Newcomer ME, Proteins. 2004 Nov 1;57(2):331-7. PMID:15340920
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