2uxf

From Proteopedia

Revision as of 11:10, 30 October 2007

PSEUDOAZURIN WITH ENGINEERED AMICYANIN LIGAND LOOP, OXIDIZED FORM, PH 5.5

Overview

Atomic resolution structures of the pseudoazurin (PAZ) variant into which, the shorter ligand-containing loop of amicyanin (AMI) is introduced have, been determined. The mutated loop adopts a different conformation in, PAZAMI than in AMI. The copper site structure is affected, with the major, influence being an increased axial interaction resulting in the shortest, Cu(II)-S(Met) bond observed for the cupredoxin family of electron-transfer, proteins. This is accompanied by a lengthening of the important Cu-S(Cys), bond and enhanced tetragonal distortion, consistent with the influence of, the PAZAMI loop contraction on the UV/vis spectrum. The change in active, site geometry is the major cause of the 50 mV decrease in reduction, potential. The copper site structure changes very little upon ... [(full description)]

About this Structure

2UXF is a [Single protein] structure of sequence from [Achromobacter cycloclastes] with CU, CL and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Influence of Loop Shortening on the Metal Binding Site of Cupredoxin Pseudoazurin(,)., Velarde M, Huber R, Yanagisawa S, Dennison C, Messerschmidt A, Biochemistry. 2007 Aug 9;. PMID:17685636

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