1ryf
From Proteopedia
(New page: 200px<br /> <applet load="1ryf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ryf, resolution 1.75Å" /> '''Alternative Splicin...) |
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| - | [[Image:1ryf. | + | [[Image:1ryf.jpg|left|200px]]<br /><applet load="1ryf" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1ryf, resolution 1.75Å" /> | caption="1ryf, resolution 1.75Å" /> | ||
'''Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase'''<br /> | '''Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RYF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] Full crystallographic information is available from [http:// | + | 1RYF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: gtp binding]] | [[Category: gtp binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:50:54 2008'' |
Revision as of 14:50, 15 February 2008
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Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase
Overview
Rac1b was recently identified in malignant colorectal tumors as an, alternative splice variant of Rac1 containing a 19-amino acid insertion, next to the switch II region. The structures of Rac1b in the GDP- and the, GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the, insertion induces an open switch I conformation and a highly mobile switch, II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP, exchange and an impaired GTP hydrolysis, which is restored partially by, GTPase-activating proteins. Interestingly, Rac1b is able to bind the, GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent, manner, suggesting that the insertion does not completely abolish effector, interaction. The presented study provides insights into the structural and, biochemical mechanism of a self-activating GTPase.
About this Structure
1RYF is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Small monomeric GTPase, with EC number 3.6.5.2 Full crystallographic information is available from OCA.
Reference
Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase., Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR, J Biol Chem. 2004 Feb 6;279(6):4743-9. Epub 2003 Nov 18. PMID:14625275
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