1s1d
From Proteopedia
(New page: 200px<br /> <applet load="1s1d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s1d, resolution 1.60Å" /> '''Structure and prote...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1s1d. | + | [[Image:1s1d.jpg|left|200px]]<br /><applet load="1s1d" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1s1d" size=" | + | |
caption="1s1d, resolution 1.60Å" /> | caption="1s1d, resolution 1.60Å" /> | ||
'''Structure and protein design of human apyrase'''<br /> | '''Structure and protein design of human apyrase'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1S1D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA, ACT, SO4, GP2 and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Apyrase Apyrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.5 3.6.1.5] Full crystallographic information is available from [http:// | + | 1S1D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=GP2:'>GP2</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Apyrase Apyrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.5 3.6.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1D OCA]. |
==Reference== | ==Reference== | ||
| Line 30: | Line 29: | ||
[[Category: nucleotide-binding motif]] | [[Category: nucleotide-binding motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:51:20 2008'' |
Revision as of 14:51, 15 February 2008
|
Structure and protein design of human apyrase
Overview
Hematophagous arthropods secrete a salivary apyrase that inhibits platelet, activation by catabolizing ADP released from damaged tissues and blood, cells. We report the X-ray crystal structures of a human enzyme of the, soluble apyrase family in its apo state and bound to a substrate analog., The structures reveal a nucleotide binding domain comprising a five-blade, beta propeller, binding determinants of the substrate and the active site, and an unusual calcium binding site with a potential regulatory function., Using a comparative structural biology approach, we were able to redesign, the human apyrase so as to enhance its ADPase activity by more than, 100-fold. The engineered enzyme is a potent inhibitor of platelet, aggregation and may serve as the basis for the development of a new class, of antithrombotic agents.
About this Structure
1S1D is a Single protein structure of sequence from Homo sapiens with , , , and as ligands. Active as Apyrase, with EC number 3.6.1.5 Full crystallographic information is available from OCA.
Reference
Structure and protein design of a human platelet function inhibitor., Dai J, Liu J, Deng Y, Smith TM, Lu M, Cell. 2004 Mar 5;116(5):649-59. PMID:15006348
Page seeded by OCA on Fri Feb 15 16:51:20 2008
Categories: Apyrase | Homo sapiens | Single protein | Dai, J. | Deng, Y. | Liu, J. | Lu, M. | Smith, T.M. | ACT | CA | GP2 | SO4 | TRS | Adpase | Calcium-binding protein | Five-blade beta propeller | Nucleotide-binding motif
