1t9z
From Proteopedia
(New page: 200px<br /> <applet load="1t9z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t9z, resolution 2.3Å" /> '''Three-dimensional st...) |
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| - | [[Image:1t9z. | + | [[Image:1t9z.jpg|left|200px]]<br /><applet load="1t9z" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1t9z" size=" | + | |
caption="1t9z, resolution 2.3Å" /> | caption="1t9z, resolution 2.3Å" /> | ||
'''Three-dimensional structure of a RNA-polymerase II binding protein.'''<br /> | '''Three-dimensional structure of a RNA-polymerase II binding protein.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http:// | + | 1T9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T9Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: alpha-beta protein]] | [[Category: alpha-beta protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:55:59 2008'' |
Revision as of 14:56, 15 February 2008
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Three-dimensional structure of a RNA-polymerase II binding protein.
Overview
Recycling of RNA polymerase II (Pol II) after transcription requires, dephosphorylation of the polymerase C-terminal domain (CTD) by the, phosphatase Fcp1. We report the X-ray structure of the small CTD, phosphatase Scp1, which is homologous to the Fcp1 catalytic domain. The, structure shows a core fold and an active center similar to those of, phosphotransferases and phosphohydrolases that solely share a DXDX(V/T), signature motif with Fcp1/Scp1. We demonstrate that the first aspartate in, the signature motif undergoes metal-assisted phosphorylation during, catalysis, resulting in a phosphoaspartate intermediate that was, structurally mimicked with the inhibitor beryllofluoride. Specificity may, result from CTD binding to a conserved hydrophobic pocket between the, active site and an insertion domain that is unique to Fcp1/Scp1. Fcp1, specificity may additionally arise from phosphatase recruitment near the, CTD via the Pol II subcomplex Rpb4/7, which is shown to be required for, binding of Fcp1 to the polymerase in vitro.
About this Structure
1T9Z is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Phosphoprotein phosphatase, with EC number 3.1.3.16 Full crystallographic information is available from OCA.
Reference
Structure and mechanism of RNA polymerase II CTD phosphatases., Kamenski T, Heilmeier S, Meinhart A, Cramer P, Mol Cell. 2004 Aug 13;15(3):399-407. PMID:15304220
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