1zip
From Proteopedia
(New page: 200px<br /> <applet load="1zip" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zip, resolution 1.85Å" /> '''BACILLUS STEAROTHER...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1ZIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]] with ZN, MN and AP5 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZIP OCA]]. | + | 1ZIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]] with ZN, MN and AP5 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3]]. Structure known Active Site: ZF. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZIP OCA]]. |
==Reference== | ==Reference== | ||
Crystal structures of Bacillus stearothermophilus adenylate kinase with bound Ap5A, Mg2+ Ap5A, and Mn2+ Ap5A reveal an intermediate lid position and six coordinate octahedral geometry for bound Mg2+ and Mn2+., Berry MB, Phillips GN Jr, Proteins. 1998 Aug 15;32(3):276-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9715904 9715904] | Crystal structures of Bacillus stearothermophilus adenylate kinase with bound Ap5A, Mg2+ Ap5A, and Mn2+ Ap5A reveal an intermediate lid position and six coordinate octahedral geometry for bound Mg2+ and Mn2+., Berry MB, Phillips GN Jr, Proteins. 1998 Aug 15;32(3):276-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9715904 9715904] | ||
| + | [[Category: Adenylate kinase]] | ||
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:17:16 2007'' |
Revision as of 11:12, 30 October 2007
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BACILLUS STEAROTHERMOPHILUS ADENYLATE KINASE
Overview
Crystal structures of Bacillus stearothermophilus adenylate kinase with, bound Ap5A, Mn2+ Ap5A, and Mg2+ Ap5A have been determined by X-ray, crystallography to resolutions of 1.6 A, 1.85 A, and 1.96 A, respectively., The protein's lid domain is partially open, being both rotated and, translated away from bound Ap5A. The flexibility of the lid domain in the, ternary state and its ability to transfer force directly to the the active, site is discussed in light of our proposed entropic mechanism for, catalytic turnover. The bound Zn2+ atom is demonstrably structural in, nature, with no contacts other than its ligating cysteine residues within, 5 A. The B. stearothermophilus adenylate kinase lid appears to be a, truncated zinc finger domain, lacking the DNA binding finger, which we, have ... [(full description)]
About this Structure
1ZIP is a [Single protein] structure of sequence from [Geobacillus stearothermophilus] with ZN, MN and AP5 as [ligands]. Active as [Adenylate kinase], with EC number [2.7.4.3]. Structure known Active Site: ZF. Full crystallographic information is available from [OCA].
Reference
Crystal structures of Bacillus stearothermophilus adenylate kinase with bound Ap5A, Mg2+ Ap5A, and Mn2+ Ap5A reveal an intermediate lid position and six coordinate octahedral geometry for bound Mg2+ and Mn2+., Berry MB, Phillips GN Jr, Proteins. 1998 Aug 15;32(3):276-88. PMID:9715904
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