1ubi
From Proteopedia
(New page: 200px<br /> <applet load="1ubi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ubi, resolution 1.8Å" /> '''SYNTHETIC STRUCTURAL...) |
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| - | [[Image:1ubi. | + | [[Image:1ubi.jpg|left|200px]]<br /><applet load="1ubi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ubi" size=" | + | |
caption="1ubi, resolution 1.8Å" /> | caption="1ubi, resolution 1.8Å" /> | ||
'''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1'''<br /> | '''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1'''<br /> | ||
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==Overview== | ==Overview== | ||
The small protein ubiquitin (76 amino acids) has been synthesized under, optimized conditions by Merrifield solid-phase methodology using the N, alpha-Fmoc protecting group. The crude polypeptide mixture was purified to, homogeneity by gel filtration, dialysis and a combination of cation- and, anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used, to authenticate the primary structure. Isoelectric focusing and m.s. were, used to demonstrate that the final product was greater than 98% pure with, a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol, scale. | The small protein ubiquitin (76 amino acids) has been synthesized under, optimized conditions by Merrifield solid-phase methodology using the N, alpha-Fmoc protecting group. The crude polypeptide mixture was purified to, homogeneity by gel filtration, dialysis and a combination of cation- and, anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used, to authenticate the primary structure. Isoelectric focusing and m.s. were, used to demonstrate that the final product was greater than 98% pure with, a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol, scale. | ||
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| + | ==Disease== | ||
| + | Known disease associated with this structure: Cleft palate, isolated OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191339 191339]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 1UBI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1UBI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UBI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: chromosomal protein]] | [[Category: chromosomal protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:00:39 2008'' |
Revision as of 15:00, 15 February 2008
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SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1
Contents |
Overview
The small protein ubiquitin (76 amino acids) has been synthesized under, optimized conditions by Merrifield solid-phase methodology using the N, alpha-Fmoc protecting group. The crude polypeptide mixture was purified to, homogeneity by gel filtration, dialysis and a combination of cation- and, anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used, to authenticate the primary structure. Isoelectric focusing and m.s. were, used to demonstrate that the final product was greater than 98% pure with, a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol, scale.
Disease
Known disease associated with this structure: Cleft palate, isolated OMIM:[191339]
About this Structure
1UBI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin., Ramage R, Green J, Muir TW, Ogunjobi OM, Love S, Shaw K, Biochem J. 1994 Apr 1;299 ( Pt 1):151-8. PMID:8166633
Page seeded by OCA on Fri Feb 15 17:00:39 2008
