1uj3
From Proteopedia
(New page: 200px<br /> <applet load="1uj3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uj3, resolution 2.10Å" /> '''Crystal structure o...) |
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caption="1uj3, resolution 2.10Å" /> | caption="1uj3, resolution 2.10Å" /> | ||
'''Crystal structure of a humanized Fab fragment of anti-tissue-factor antibody in complex with tissue factor'''<br /> | '''Crystal structure of a humanized Fab fragment of anti-tissue-factor antibody in complex with tissue factor'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UJ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1UJ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJ3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tissue factor]] | [[Category: tissue factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:02:32 2008'' |
Revision as of 15:02, 15 February 2008
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Crystal structure of a humanized Fab fragment of anti-tissue-factor antibody in complex with tissue factor
Contents |
Overview
Tissue factor (TF) is a membrane-anchored protein that initiates the, extrinsic cascade of blood coagulation. TF forms a complex with serine, protease Factor VIIa, and then activates Factor X zymogen to Factor Xa, leading to the blood coagulation. Humanized anti-TF antibody hATR-5, strongly inhibits TF-initiated blood coagulation, and is of potential use, for various thrombotic diseases. The Fab fragment of antibody hATR-5 is, obtained for crystallization. The crystal structure of the complex of the, Fab with extracellular domains of human TF was determined with the, molecular replacement method, and refined to an R factor of 0.196 at 2.1 A, resolution. All the complementarity-determining regions (CDRs) of the Fab, are involved in interaction with the C-terminal-side extracellular domain, of TF through 19 hydrogen bonds. The interface between the Fab and TF, molecules contains 15 water molecules, and yields buried surface areas as, wide as 2000 A2. The TF surface in the interface is possibly involved in, the activation of Factor X, by forming a transient ternary complex of, Factor X-TF-Factor VIIa. Electrostatic interactions are predominantly, observed between the heavy-chain CDRs and TF. These hydrogen-bonding and, electrostatic interactions together with the wide buried areas contribute, to the high affinity of the antibody toward TF, leading to the effective, inhibition of the TF-initiated blood coagulation.
Disease
Known disease associated with this structure: Esophageal squamous cell carcinoma OMIM:[606551]
About this Structure
1UJ3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a humanized Fab fragment of anti-tissue-factor antibody in complex with tissue factor., Ohto U, Mizutani R, Nakamura M, Adachi H, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):105-8. Epub 2003 Nov 28. PMID:14646147
Page seeded by OCA on Fri Feb 15 17:02:32 2008
Categories: Homo sapiens | Single protein | Adachi, H. | Mizutani, R. | Nakamura, M. | Ohto, U. | Satow, Y. | Antibody | Antigen | Fab | Tissue factor
