1gtf
From Proteopedia
(New page: 200px<br /> <applet load="1gtf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gtf, resolution 1.750Å" /> '''THE STRUCTURE OF T...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GTF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ | + | 1GTF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]] with TRP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Sites: AC1, AC2, AC3, AC4, AC5, AC6, AC7, AC8, AC9, BC1, BC2, BC3, BC4, BC5, BC6, BC7, BC8, BC9, CC1, CC2, CC3 and CC4. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GTF OCA]]. |
==Reference== | ==Reference== | ||
Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11914485 11914485] | Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11914485 11914485] | ||
| - | [[Category: | + | [[Category: Geobacillus stearothermophilus]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Antson, A.A.]] | [[Category: Antson, A.A.]] | ||
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[[Category: trp rna]] | [[Category: trp rna]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:17:56 2007'' |
Revision as of 11:13, 30 October 2007
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THE STRUCTURE OF THE TRP RNA-BINDING ATTENUATION PROTEIN (TRAP) BOUND TO A 53-NUCLEOTIDE RNA MOLECULE CONTAINING GAGUU REPEATS
Overview
The trp RNA-binding attenuation protein (TRAP) regulates expression of the, tryptophan biosynthetic genes in bacilli by binding to the leader region, of the nascent trp operon mRNA. When activated by binding tryptophan, the, 11-subunit circular TRAP molecule binds to a target sequence consisting of, 11 (G/U)AG repeats, separated by two or three variable 'spacer', nucleotides. Reported here are two crystal structures of TRAP bound to, RNAs containing 11 GAG repeats separated by UU and CC spacer nucleotides, determined at 1.75 and 2.50 A resolution, respectively. These show the, spacer regions of the RNA molecules to be highly flexible, making no, direct hydrogen-bonding contacts with the protein. Comparison of these, structures with the previous structure of TRAP bound to (GAGAU)(10)GAG, ... [(full description)]
About this Structure
1GTF is a [Single protein] structure of sequence from [Geobacillus stearothermophilus] with TRP as [ligand]. Structure known Active Sites: AC1, AC2, AC3, AC4, AC5, AC6, AC7, AC8, AC9, BC1, BC2, BC3, BC4, BC5, BC6, BC7, BC8, BC9, CC1, CC2, CC3 and CC4. Full crystallographic information is available from [OCA].
Reference
Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences., Hopcroft NH, Wendt AL, Gollnick P, Antson AA, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):615-21. Epub 2002, Mar 22. PMID:11914485
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