Sandbox Reserved 564

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(New page: <!-- PLEASE DO NOT DELETE THIS TEMPLATE --> {{NCSU_BIOL414_Sandbox}} <!-- PLEASE ADD YOUR CONTENT BELOW HERE --> Hi everyone, Here is your very own Proteopedia Page to play with. This page...)
Current revision (18:12, 24 May 2012) (edit) (undo)
 
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{{NCSU_BIOL414_Sandbox}}
{{NCSU_BIOL414_Sandbox}}
<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
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Hi everyone,
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Hi Mike,
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Here is your very own Proteopedia Page to play with.
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Here is your very own Proteopedia Page for pdb code: 1M8Q.
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This page is not publicly available yet,
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Your presentation is scheduled for: June 15.
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so don't worry about making mistakes that
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other people will see.
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Have Fun!
Have Fun!
Greg Buhrman
Greg Buhrman
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[[Image:1m8q.png|left|200px]]
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The line below this paragraph, containing "STRUCTURE_1m8q", creates the "Structure Box" on the page.
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{{STRUCTURE_1m8q| PDB=1m8q | SCENE= }}
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===Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle===
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{{ABSTRACT_PUBMED_12160705}}
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==About this Structure==
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[[1m8q]] is a 26 chain structure of [[Actin]] and [[Myosin]] with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8Q OCA].
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==See Also==
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*[[Actin]]
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*[[Myosin]]
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==Reference==
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<ref group="xtra">PMID:12160705</ref><references group="xtra"/>
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[[Category: Gallus gallus]]
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[[Category: Oryctolagus cuniculus]]
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[[Category: Chen, L F.]]
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[[Category: Reedy, M C.]]
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[[Category: Reedy, M K.]]
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[[Category: Taylor, K A.]]
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[[Category: Winkler, H.]]
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[[Category: Actin-myosin complex in situ in muscle]]
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[[Category: Contractile protein]]

Current revision

This Sandbox is Reserved from 05/22/2012, through 07/22/2012 for use in the course "BIOL 414" taught by Greg Buhrman at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 551 through Sandbox Reserved 590.
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • Click the 3D button (when editing, above the wikitext box) to insert Jmol.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing

Hi Mike, Here is your very own Proteopedia Page for pdb code: 1M8Q. Your presentation is scheduled for: June 15.

Have Fun!

Greg Buhrman


PDB ID 1m8q

Drag the structure with the mouse to rotate
1m8q, resolution 70.00Å ()
Non-Standard Residues:
Related: 2mys, 1atn
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle

Publication Abstract from PubMed

Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.

Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle., Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA, J Struct Biol. 2002 Apr-May;138(1-2):92-104. PMID:12160705

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1m8q is a 26 chain structure of Actin and Myosin with sequence from Gallus gallus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.

See Also

Reference

  • Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA. Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle. J Struct Biol. 2002 Apr-May;138(1-2):92-104. PMID:12160705
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