Sandbox Reserved 565

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(New page: <!-- PLEASE DO NOT DELETE THIS TEMPLATE --> {{NCSU_BIOL414_Sandbox}} <!-- PLEASE ADD YOUR CONTENT BELOW HERE --> Hi everyone, Here is your very own Proteopedia Page to play with. This page...)
Current revision (18:15, 24 May 2012) (edit) (undo)
 
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{{NCSU_BIOL414_Sandbox}}
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<!-- PLEASE ADD YOUR CONTENT BELOW HERE -->
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Hi everyone,
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Hi Eric,
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Here is your very own Proteopedia Page to play with.
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Here is your very own Proteopedia Page for pdb code: 2NCD.
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This page is not publicly available yet,
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Your presentation is scheduled for: June 14.
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so don't worry about making mistakes that
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other people will see.
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Have Fun!
Have Fun!
Greg Buhrman
Greg Buhrman
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[[Image:2ncd.png|left|200px]]
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The line below this paragraph, containing "STRUCTURE_2ncd", creates the "Structure Box" on the page.
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{{STRUCTURE_2ncd| PDB=2ncd | SCENE= }}
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===NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER===
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The line below this paragraph, {{ABSTRACT_PUBMED_9796817}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_9796817}}
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==About this Structure==
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[[2ncd]] is a 1 chain structure of [[Kinesin]] with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. The April 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Kinesin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_4 10.2210/rcsb_pdb/mom_2005_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NCD OCA].
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==See Also==
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*[[Kinesin]]
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==Reference==
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<ref group="xtra">PMID:9796817</ref><references group="xtra"/>
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[[Category: Drosophila melanogaster]]
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[[Category: Kinesin]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Case, R B.]]
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[[Category: Dai, S C.]]
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[[Category: Fletterick, R J.]]
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[[Category: Hart, C L.]]
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[[Category: Ruby, A.]]
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[[Category: Sablin, E P.]]
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[[Category: Vale, R D.]]
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[[Category: Contractile protein]]
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[[Category: Kinesin]]
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[[Category: Microtubule-based motor]]
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[[Category: Ncd]]

Current revision

This Sandbox is Reserved from 05/22/2012, through 07/22/2012 for use in the course "BIOL 414" taught by Greg Buhrman at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 551 through Sandbox Reserved 590.
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • Click the 3D button (when editing, above the wikitext box) to insert Jmol.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing

Hi Eric, Here is your very own Proteopedia Page for pdb code: 2NCD. Your presentation is scheduled for: June 14.

Have Fun!

Greg Buhrman


PDB ID 2ncd

Drag the structure with the mouse to rotate
2ncd, resolution 2.50Å ()
Ligands: ,
Gene: NCD (Drosophila melanogaster)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER

Publication Abstract from PubMed

Motor proteins of the kinesin superfamily transport intracellular cargo along microtubules. Although different kinesin proteins share 30-50% amino-acid identity in their motor catalytic cores, some move to the plus end of microtubules whereas others travel in the opposite direction. Crystal structures of the catalytic cores of conventional kinesin (a plus-end-directed motor involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregation) are nearly identical; therefore, the structural basis for their opposite directions of movement is unknown. Here we show that the ncd 'neck' made up of 13 class-specific residues next to the superfamily-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these neck residues reverses the direction of ncd motion. By solving the 2.5 A structure of a functional ncd dimer, we show that the ncd neck (a coiled-coil) differs from the corresponding region in the kinesin neck (an interrupted beta-strand), although both necks interact with similar elements in the catalytic cores. The distinct neck architectures also confer different symmetries to the ncd and kinesin dimers and position these motors with appropriate directional bias on the microtubule.

Direction determination in the minus-end-directed kinesin motor ncd., Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ, Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2ncd is a 1 chain structure of Kinesin with sequence from Drosophila melanogaster. The April 2005 RCSB PDB Molecule of the Month feature on Kinesin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_4. Full crystallographic information is available from OCA.

See Also

Reference

  • Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ. Direction determination in the minus-end-directed kinesin motor ncd. Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817 doi:10.1038/27463
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