1tez
From Proteopedia
(New page: 200px<br /> <applet load="1tez" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tez, resolution 1.80Å" /> '''COMPLEX BETWEEN DNA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1TEZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]] with MG, FAD and HDF as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA]]. | + | 1TEZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]] with MG, FAD and HDF as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15576622 15576622] | Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15576622 15576622] | ||
| + | [[Category: Deoxyribodipyrimidine photo-lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Synechococcus sp.]] | [[Category: Synechococcus sp.]] | ||
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[[Category: photolyase; dna repair; light-driven electron transfer]] | [[Category: photolyase; dna repair; light-driven electron transfer]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:19:05 2007'' |
Revision as of 11:14, 30 October 2007
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COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS
Overview
DNA photolyases use light energy to repair DNA that comprises, ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine, dimers (CPDs). Here we report the crystal structure of a DNA photolyase, bound to duplex DNA that is bent by 50 degrees and comprises a synthetic, CPD lesion. This CPD lesion is flipped into the active site and split, there into two thymines by synchrotron radiation at 100 K. Although, photolyases catalyze blue light-driven CPD cleavage only above 200 K, this, structure apparently mimics a structural substate during light-driven DNA, repair in which back-flipping of the thymines into duplex DNA has not yet, taken place.
About this Structure
1TEZ is a [Single protein] structure of sequence from [Synechococcus sp.] with MG, FAD and HDF as [ligands]. Active as [Deoxyribodipyrimidine photo-lyase], with EC number [4.1.99.3]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622
Page seeded by OCA on Tue Oct 30 13:19:05 2007
