1a5s
From Proteopedia
(New page: 200px<br /> <applet load="1a5s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a5s, resolution 2.30Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1A5S is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, FIP, PLP and SER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5S OCA]]. | + | 1A5S is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, FIP, PLP and SER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Sites: S1 and S2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5S OCA]]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
| + | [[Category: Tryptophan synthase]] | ||
[[Category: Anderson, K.S.]] | [[Category: Anderson, K.S.]] | ||
[[Category: Gerhardt, E.]] | [[Category: Gerhardt, E.]] | ||
| Line 30: | Line 31: | ||
[[Category: tryptophan biosynthesis]] | [[Category: tryptophan biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:19:13 2007'' |
Revision as of 11:14, 30 October 2007
|
CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH 5-FLUOROINDOLE PROPANOL PHOSPHATE AND L-SER BOUND AS AMINO ACRYLATE TO THE BETA SITE
Overview
Crystal structures of wild-type tryptophan synthase alpha2beta2 complexes, from Salmonella typhimurium were determined to investigate the mechanism, of allosteric activation of the alpha-reaction by the aminoacrylate, intermediate formed at the beta-active site. Using a flow cell, the, aminoacrylate (A-A) intermediate of the beta-reaction () was generated in, the crystal under steady state conditions in the presence of serine and, the alpha-site inhibitor 5-fluoroindole propanol phosphate (F-IPP). A, model for the conformation of the Schiff base between the aminoacrylate, and the beta-subunit cofactor pyridoxal phosphate (PLP) is presented. The, structure is compared with structures of the enzyme determined in the, absence (TRPS) and presence (TRPSF-IPP) of F-IPP. A detailed model for, ... [(full description)]
About this Structure
1A5S is a [Protein complex] structure of sequences from [Salmonella typhimurium] with NA, FIP, PLP and SER as [ligands]. Active as [Tryptophan synthase], with EC number [4.2.1.20]. Structure known Active Sites: S1 and S2. Full crystallographic information is available from [OCA].
Reference
Loop closure and intersubunit communication in tryptophan synthase., Schneider TR, Gerhardt E, Lee M, Liang PH, Anderson KS, Schlichting I, Biochemistry. 1998 Apr 21;37(16):5394-406. PMID:9548921
Page seeded by OCA on Tue Oct 30 13:19:13 2007
