1xna
From Proteopedia
(New page: 200px<br /> <applet load="1xna" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xna" /> '''NMR SOLUTION STRUCTURE OF THE SINGLE-STRAND...) |
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'''NMR SOLUTION STRUCTURE OF THE SINGLE-STRAND BREAK REPAIR PROTEIN XRCC1-N-TERMINAL DOMAIN'''<br /> | '''NMR SOLUTION STRUCTURE OF THE SINGLE-STRAND BREAK REPAIR PROTEIN XRCC1-N-TERMINAL DOMAIN'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XNA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1XNA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xrcc1]] | [[Category: xrcc1]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:09:44 2008'' |
Revision as of 15:09, 15 February 2008
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NMR SOLUTION STRUCTURE OF THE SINGLE-STRAND BREAK REPAIR PROTEIN XRCC1-N-TERMINAL DOMAIN
Overview
XRCC1 functions in the repair of single-strand DNA breaks in mammalian, cells and forms a repair complex with beta-Pol, ligase III and PARP. Here, we describe the NMR solution structure of the XRCC1 N-terminal domain, (XRCC1 NTD). The structural core is a beta-sandwich with beta-strands, connected by loops, three helices and two short two-stranded beta-sheets, at each connection side. We show, for the first time, that the XRCC1 NTD, specifically binds single-strand break DNA (gapped and nicked). We also, show that the XRCC1 NTD binds a gapped DNA-beta-Pol complex. The DNA, binding and beta-Pol binding surfaces were mapped by NMR and found to be, well suited for interaction with single-strand gap DNA containing a 90, degrees bend, and for simultaneously making contacts with the palm-thumb, of beta-Pol in a ternary complex. The findings suggest a mechanism for, preferential binding of the XRCC1 NTD to flexible single-strand break DNA.
About this Structure
1XNA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the single-strand break repair protein XRCC1 N-terminal domain., Marintchev A, Mullen MA, Maciejewski MW, Pan B, Gryk MR, Mullen GP, Nat Struct Biol. 1999 Sep;6(9):884-93. PMID:10467102
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