2a8i
From Proteopedia
(New page: 200px<br /> <applet load="2a8i" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a8i, resolution 2.00Å" /> '''Crystal Structure o...) |
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caption="2a8i, resolution 2.00Å" /> | caption="2a8i, resolution 2.00Å" /> | ||
'''Crystal Structure of human Taspase1'''<br /> | '''Crystal Structure of human Taspase1'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A8I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2A8I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: threonine aspartase]] | [[Category: threonine aspartase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:14:03 2008'' |
Revision as of 15:14, 15 February 2008
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Crystal Structure of human Taspase1
Overview
Taspase1 catalyzes the proteolytic processing of the mixed lineage, leukemia (MLL) nuclear protein, which is required for maintaining Hox gene, expression patterns. Chromosomal translocations of the MLL gene are, associated with leukemia in infants. Taspase1, a threonine aspartase, is a, member of the type 2 asparaginase family, but is the only protease in this, family. We report here the crystal structures of human activated Taspase1, and its proenzyme, as well as the characterization of the effects of, mutations in the active site region using a newly developed fluorogenic, assay. The structure of Taspase1 has significant differences from other, asparaginases, especially near the active site. Mutation of the catalytic, nucleophile, Thr234, abolishes autocatalytic processing in cis but does, not completely block proteolysis in trans. The structure unexpectedly, showed the binding of a chloride ion in the active site, and our kinetic, studies confirm that chlorides ions are inhibitors of this enzyme at, physiologically relevant concentrations.
About this Structure
2A8I is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52. PMID:16216576
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