1up3

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(New page: 200px<br /> <applet load="1up3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1up3, resolution 1.60&Aring;" /> '''STRUCTURE OF THE EN...)
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==About this Structure==
==About this Structure==
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1UP3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with SO4 and 1PG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UP3 OCA]].
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1UP3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with SO4 and 1PG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UP3 OCA]].
==Reference==
==Reference==
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[[Category: glycoside hydrolase]]
[[Category: glycoside hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:29:34 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:20:57 2007''

Revision as of 11:16, 30 October 2007


1up3, resolution 1.60Å

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STRUCTURE OF THE ENDOGLUCANASE CEL6 FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH METHYL-CELLOBIOSYL-4-DEOXY-4-THIO-BETA-D-CELLOBIOSIDE AT 1.6 ANGSTROM

Overview

The genomes of various Mycobacterium tuberculosis strains encode proteins, that do not appear to play a role in the growth or survival of the, bacterium in its mammalian host, including some implicated in plant cell, wall breakdown. Here we show that M. tuberculosis H37Rv does indeed, possess a functional cellulase. The x-ray crystal structure of this, enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a, highly conserved substrate-binding cleft, which affords similar, and, unusual, distortion of the substrate at the catalytic center. The, endoglucanase activity, together with the existence of a putative, membrane-associated crystalline polysaccharide-binding protein, may, reflect the ancestral soil origin of the Mycobacterium or hint at a, previously unconsidered ... [(full description)]

About this Structure

1UP3 is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with SO4 and 1PG as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Mycobacterium tuberculosis strains possess functional cellulases., Varrot A, Leydier S, Pell G, Macdonald JM, Stick RV, Henrissat B, Gilbert HJ, Davies GJ, J Biol Chem. 2005 May 27;280(21):20181-4. Epub 2005 Apr 11. PMID:15824123

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