2znb
From Proteopedia
(New page: 200px<br /> <applet load="2znb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2znb, resolution 2.15Å" /> '''METALLO-BETA-LACTAM...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2ZNB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]] with CD and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ZNB OCA]]. | + | 2ZNB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteroides_fragilis Bacteroides fragilis]] with CD and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Structure known Active Sites: CD1, CD2 and NA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ZNB OCA]]. |
==Reference== | ==Reference== | ||
Crystal structures of the cadmium- and mercury-substituted metallo-beta-lactamase from Bacteroides fragilis., Concha NO, Rasmussen BA, Bush K, Herzberg O, Protein Sci. 1997 Dec;6(12):2671-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9416622 9416622] | Crystal structures of the cadmium- and mercury-substituted metallo-beta-lactamase from Bacteroides fragilis., Concha NO, Rasmussen BA, Bush K, Herzberg O, Protein Sci. 1997 Dec;6(12):2671-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9416622 9416622] | ||
[[Category: Bacteroides fragilis]] | [[Category: Bacteroides fragilis]] | ||
| + | [[Category: Beta-lactamase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Concha, N.O.]] | [[Category: Concha, N.O.]] | ||
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[[Category: metallo beta-lactamase]] | [[Category: metallo beta-lactamase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:22:35 2007'' |
Revision as of 11:17, 30 October 2007
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METALLO-BETA-LACTAMASE (CADMIUM-BOUND FORM)
Overview
The metallo-beta-lactamases require zinc or cadmium for hydrolyzing, beta-lactam antibiotics and are inhibited by mercurial compounds. To data, there are no clinically useful inhibitors of this class of enzymes. The, crystal structure of the Zn(2+)-bound enzyme from Bacteroides fragilis, contains a binuclear zinc center in the active site. A hydroxide, coordinated to both zinc atoms, is proposed as the moiety that mounts the, nucleophilic attack on the carbonyl carbon atom of the beta-lactam ring., To study the metal coordination further, the crystal structures of a, Cd(2+)-bound enzyme and of an Hg(2+)-soaked zinc-containing enzyme have, been determined at 2.1 A and 2.7 A, respectively. Given the diffraction, resolution, the Cd(2+)-bound enzyme exhibits the same active-site, architecture ... [(full description)]
About this Structure
2ZNB is a [Single protein] structure of sequence from [Bacteroides fragilis] with CD and NA as [ligands]. Active as [Beta-lactamase], with EC number [3.5.2.6]. Structure known Active Sites: CD1, CD2 and NA. Full crystallographic information is available from [OCA].
Reference
Crystal structures of the cadmium- and mercury-substituted metallo-beta-lactamase from Bacteroides fragilis., Concha NO, Rasmussen BA, Bush K, Herzberg O, Protein Sci. 1997 Dec;6(12):2671-6. PMID:9416622
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