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The Crystal Structure of the Protein of Unknown Function VCA0042 from Vibrio cholerae O1 (1yln)
(R136, R140, D162, S164, G167)
1YLN is a 1 chain structure of sequence from Vibrio cholerae o1. Full crystallographic information is available from OCA.
Solution NMR structure of Pseudomonas Aeruginosa protein PA4608. Northeast Structural Genomics target PaT7 (1ywu)
Publication Abstract from PubMed
PA4608 is a 125 residue protein from Pseudomonas aeruginosa with a recent identification as a PilZ domain and putative bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) adaptor protein that plays a role in bacterial second-messenger regulated processes. The nuclear magnetic resonance (NMR) structure of PA4608 has been determined and c-di-GMP binding has been confirmed by NMR titration studies. The monomeric core structure of PA4608 contains a six-stranded anti-parallel beta barrel flanked by three helices. Conserved surface residues among PA4608 homologs suggest the c-di-GMP binding site is at one end of the barrel and includes residues in the helices as well as in the unstructured N-terminus. Chemical shift changes in PA4608 resonances upon titration with c-di-GMP confirm binding. This evidence supports the hypothesis that proteins containing PilZ domains are the long-sought c-di-GMP adaptor proteins.
NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa is a PilZ domain and a c-di-GMP binding protein., Ramelot TA, Yee A, Cort JR, Semesi A, Arrowsmith CH, Kennedy MA, Proteins. 2007 Feb 1;66(2):266-71. PMID:17096419
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
(R9, R13, D35, S37, G40)
Crystal structure of predicted glycosyltransferase from Pseudomonas putida KT2440 at 2.25 A resolution (2gjg)
(R123xxR, D157xSxxG)
(R123, R127, D157, S159, G162)
2GJG is a 1 chain structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Created by Tilman Schirmer.
