2erm
From Proteopedia
(New page: 200px<br /> <applet load="2erm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2erm" /> '''Solution structure of a biologically active...) |
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'''Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue'''<br /> | '''Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ERM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with IPA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2ERM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-carbohydrate complex]] | [[Category: protein-carbohydrate complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:23:46 2008'' |
Revision as of 15:23, 15 February 2008
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Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue
Contents |
Overview
The 3D structure of a complex formed by the acidic fibroblast growth, factor (FGF-1) and a specifically designed synthetic heparin, hexasaccharide has been determined by NMR spectroscopy. This, hexasaccharide can substitute natural heparins in FGF-1 mitogenesis, assays, in spite of not inducing any apparent dimerization of the growth, factor. The use of this well defined synthetic heparin analogue has, allowed us to perform a detailed NMR structural analysis of the, heparin-FGF interaction, overcoming the limitations of NMR to deal with, the high molecular mass and heterogeneity of the FGF-1 oligomers formed in, the presence of natural heparin fragments. Our results confirm that, glycosaminoglycans induced FGF-1 dimerization either in a cis or trans, disposition with respect to the heparin chain is not an absolute, requirement for biological activity.
Disease
Known diseases associated with this structure: Aplasia of lacrimal and salivary glands OMIM:[602115], LADD syndrome OMIM:[602115]
About this Structure
2ERM is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue., Canales A, Lozano R, Lopez-Mendez B, Angulo J, Ojeda R, Nieto PM, Martin-Lomas M, Gimenez-Gallego G, Jimenez-Barbero J, FEBS J. 2006 Oct;273(20):4716-27. Epub 2006 Sep 21. PMID:16995857
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