1gnz

From Proteopedia

Revision as of 11:18, 30 October 2007

LECTIN I-B4 FROM GRIFFONIA SIMPLICIFOLIA (GS I-B4)METAL FREE FORM

Overview

Seeds from the African legume shrub Griffonia simplicifolia contain, several lectins. Among them the tetrameric lectin GS I-B(4) has strict, specificity for terminal alpha Gal residues, whereas the closely related, lectin GS I-A(4) can also bind to alpha GalNAc. These two lectins are, commonly used as markers in histology or for research in, xenotransplantation. To elucidate the basis for the fine difference in, specificity, the amino acid sequences of both lectins have been determined, and show 89% identity. The crystal structure of GS I-B(4), determined at, 2.5-A resolution, reveals a new quaternary structure that has never been, observed in other legume lectins. An unexpected loss of both Ca(2+) and, Mn(2+) ions, which are necessary for carbohydrate binding in legume, lectins, may be ... [(full description)]

About this Structure

1GNZ is a [Single protein] structure of sequence from [Griffonia simplicifolia] with NAG and PO4 as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Isolectins I-A and I-B of Griffonia (Bandeiraea) simplicifolia. Crystal structure of metal-free GS I-B(4) and molecular basis for metal binding and monosaccharide specificity., Lescar J, Loris R, Mitchell E, Gautier C, Chazalet V, Cox V, Wyns L, Perez S, Breton C, Imberty A, J Biol Chem. 2002 Feb 22;277(8):6608-14. Epub 2001 Nov 19. PMID:11714720

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