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Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription (3dmq)
Publication Abstract from PubMed
RapA, as abundant as sigma70 in the cell, is an RNA polymerase (RNAP)-associated Swi2/Snf2 protein with ATPase activity. It stimulates RNAP recycling during transcription. We report a structure of RapA that is also a full-length structure for the entire Swi2/Snf2 family. RapA contains seven domains, two of which exhibit novel protein folds. Our model of RapA in complex with ATP and double-stranded DNA (dsDNA) suggests that RapA may bind to and translocate on dsDNA. Our kinetic template-switching assay shows that RapA facilitates the release of sequestered RNAP from a posttranscrption/posttermination complex for transcription reinitiation. Our in vitro competition experiment indicates that RapA binds to core RNAP only but is readily displaceable by sigma70. RapA is likely another general transcription factor, the structure of which provides a framework for future studies of this bacterial Swi2/Snf2 protein and its important roles in RNAP recycling during transcription.
Structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription., Shaw G, Gan J, Zhou YN, Zhi H, Subburaman P, Zhang R, Joachimiak A, Jin DJ, Ji X, Structure. 2008 Sep 10;16(9):1417-27. PMID:18786404
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
Template:STRUCTURE 3dmq
3dmq is a 2 chains structure of sequences from Escherichia coli k12. Full crystallographic information is available from OCA.
The .
The structure of each are colored according to Figure 2 of the paper describing the structure. as in Figure 2. .
; the two subdomains are referred to as NtdA and NtdB. The Tudor-like fold is also seen in the transcription factors NusG (1npr,1npp,1m1h,1m1g) and Mfd (TRCF, transcription-repair coupling factor -2eyq), as well as ribosomal protein L24 (1jj2), human survival of motor neuron protein (1g5v), mammalian DNA repair factor 53BP1 (2ig0,2g3r), and putative fission yeast DNA repair factor Crb2 (2fhd).
represented similar to Figure 1 of the paper describing the structure.
[Note: the following view generates a substantial surface which may take several minutes to calculate. Use one above as an alternative unless you are willing to spend the time. ] represented much more similar to Figure 1 of the paper describing the structure. .
with the motifs colored as in figure 3 of the paper describing the structure. ( with the motifs colored and the backbone shown as in figure 3 of the paper describing the structure.)
in the structure. . .
in the context of the entire structure. .
represented similarly to a portion of Figure 1 of the paper describing the structure. . .
represented similarly to figure 4 of the paper describing the structure. .
[Note: the following view generates a substantial surface which may take several minutes to calculate. Use one above as an alternative unless you are willing to spend the time.] represented very close to how it is illustrated in figure 4 of the paper describing the structure. .
Reference
- Shaw G, Gan J, Zhou YN, Zhi H, Subburaman P, Zhang R, Joachimiak A, Jin DJ, Ji X. Structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription. Structure. 2008 Sep 10;16(9):1417-27. PMID:18786404 doi:10.1016/j.str.2008.06.012
