RapA, a Swi2/Snf2 protein

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Revision as of 13:21, 11 June 2012

Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription (3dmq)

Publication Abstract from PubMed

RapA, as abundant as sigma70 in the cell, is an RNA polymerase (RNAP)-associated Swi2/Snf2 protein with ATPase activity. It stimulates RNAP recycling during transcription. We report a structure of RapA that is also a full-length structure for the entire Swi2/Snf2 family. RapA contains seven domains, two of which exhibit novel protein folds. Our model of RapA in complex with ATP and double-stranded DNA (dsDNA) suggests that RapA may bind to and translocate on dsDNA. Our kinetic template-switching assay shows that RapA facilitates the release of sequestered RNAP from a posttranscrption/posttermination complex for transcription reinitiation. Our in vitro competition experiment indicates that RapA binds to core RNAP only but is readily displaceable by sigma70. RapA is likely another general transcription factor, the structure of which provides a framework for future studies of this bacterial Swi2/Snf2 protein and its important roles in RNAP recycling during transcription.

Structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription., Shaw G, Gan J, Zhou YN, Zhi H, Subburaman P, Zhang R, Joachimiak A, Jin DJ, Ji X, Structure. 2008 Sep 10;16(9):1417-27. PMID:18786404

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3dmq is a 2 chains structure of sequences from Escherichia coli k12. Full crystallographic information is available from OCA.

The .

The structure of each are colored according to Figure 2 of the paper describing the structure. as in Figure 2. .

; the two subdomains are referred to as NtdA and NtdB. The Tudor-like fold is also seen in the transcription factors NusG (1npr,1npp,1m1h,1m1g) and Mfd (TRCF, transcription-repair coupling factor -2eyq), as well as ribosomal protein L24 (1jj2), human survival of motor neuron protein (1g5v), mammalian DNA repair factor 53BP1 (2ig0,2g3r), and putative fission yeast DNA repair factor Crb2 (2fhd).

represented similar to Figure 1 of the paper describing the structure.

[Note: the following view generates a substantial surface which may take several minutes to calculate. Use one above as an alternative unless you are willing to spend the time. ] represented much more similar to Figure 1 of the paper describing the structure. .

with the motifs colored as in figure 3 of the paper describing the structure. ( with the motifs colored and the backbone shown as in figure 3 of the paper describing the structure.)

in the structure. . .

in the context of the entire structure. .

represented similarly to a portion of Figure 1 of the paper describing the structure. . .

represented similarly to figure 4 of the paper describing the structure. .

[Note: the following view generates a substantial surface which may take several minutes to calculate. Use one above as an alternative unless you are willing to spend the time.] represented very close to how it is illustrated in figure 4 of the paper describing the structure. .

Reference

Shaw G, Gan J, Zhou YN, Zhi H, Subburaman P, Zhang R, Joachimiak A, Jin DJ, Ji X. Structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription. Structure. 2008 Sep 10;16(9):1417-27. PMID:18786404 doi:10.1016/j.str.2008.06.012

Created with the participation of Wayne Decatur.

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel

Retrieved from "http://52.214.119.220/wiki/index.php/RapA%2C_a_Swi2/Snf2_protein"

@@ Line 1: / Line 1: @@
-<StructureSection load="3dmq" size="400" color="" frame="true"  spin="on" Scene='3dmq/Rapaasym/4'  align="right" caption='3dmq asymmetric unit, resolution 3.20&Aring;. <br> Click <scene name='3dmq/Rapa/1'>here</scene> to display biological molecule' >
+<StructureSection load="3dmq" size="400" color="" frame="true"  spin="on" Scene='3dmq/Rapaasym/4'  align="right" caption= >
 [[Image:3dmq.png|left|200px]]
+===Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription (3dmq)===
-===Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription (3dmq)===
-<!--
-The line below this paragraph, {{ABSTRACT_PUBMED_18786404}}, adds the Publication Abstract to the page
-(as it appears on PubMed at http://www.pubmed.gov), where 18786404 is the PubMed ID number.
--->
 {{ABSTRACT_PUBMED_18786404}}
 ==About this Structure==
-{{STRUCTURE_3dmq|  PDB=3dmq  |  SCENE=3dmq/Rapaasym/4  |CAPTION= 3dmq asymmetric unit, resolution 3.20&Aring;. <br> Click <scene name='3dmq/Rapa/1'>here</scene> to display biological molecule.}}
 dmq is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DMQ OCA].
 <scene name='3dmq/Rapaasym/4'>The crystallographic asymmetric unit of the solved structure contains 2 monomers of RapA.</scene> {{Link Toggle RapASulfate}}
@@ Line 76: / Line 69: @@
 <nowiki>[</nowiki>Note: the following view generates a substantial surface which may take several minutes to calculate. Use one above as an alternative unless you are willing to spend the time.<nowiki>]</nowiki><scene name='3dmq/Rapaatpasesurf/1'>ATPase core</scene> represented very close to how it is illustrated in [http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6VSR-4TDD114-K&_image=fig4&_ba=4&_user=10&_rdoc=1&_fmt=full&_orig=search&_cdi=6269&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=4598acd6991f7224abc57c04e194d0b8 figure 4] of the paper describing the structure. {{Link Toggle RapASulfate}}.
+</StructureSection>
 ==Reference==
 <ref group="xtra">PMID:18786404</ref><references group="xtra"/>
@@ Line 103: / Line 96: @@
 [[Category: Transcription regulation]]
 [[Category: Sf2 atpase]]
+<br />
+Created with the participation of [[User:Wayne Decatur|Wayne Decatur]].

RapA, a Swi2/Snf2 protein

From Proteopedia

Revision as of 13:21, 11 June 2012

Crystal structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription (3dmq)

About this Structure

Reference

Proteopedia Page Contributors and Editors (what is this?)

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