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2ghw
From Proteopedia
(New page: 200px<br /> <applet load="2ghw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ghw, resolution 2.30Å" /> '''Crystal structure o...) |
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| - | [[Image:2ghw.gif|left|200px]]<br /> | + | [[Image:2ghw.gif|left|200px]]<br /><applet load="2ghw" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2ghw" size=" | + | |
caption="2ghw, resolution 2.30Å" /> | caption="2ghw, resolution 2.30Å" /> | ||
'''Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R'''<br /> | '''Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GHW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GHW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sars]] | [[Category: sars]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:28:02 2008'' |
Revision as of 15:28, 15 February 2008
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Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R
Overview
Severe acute respiratory syndrome (SARS) is a newly emerged infectious, disease that caused pandemic spread in 2003. The etiological agent of SARS, is a novel coronavirus (SARS-CoV). The coronaviral surface spike protein S, is a type I transmembrane glycoprotein that mediates initial host binding, via the cell surface receptor angiotensin-converting enzyme 2 (ACE2), as, well as the subsequent membrane fusion events required for cell entry., Here we report the crystal structure of the S1 receptor binding domain, (RBD) in complex with a neutralizing antibody, 80R, at 2.3 A resolution, as well as the structure of the uncomplexed S1 RBD at 2.2 A resolution. We, show that the 80R-binding epitope on the S1 RBD overlaps very closely with, the ACE2-binding site, providing a rationale for the strong binding and, broad neutralizing ability of the antibody. We provide a structural basis, for the differential effects of certain mutations in the spike protein on, 80R versus ACE2 binding, including escape mutants, which should facilitate, the design of immunotherapeutics to treat a future SARS outbreak. We, further show that the RBD of S1 forms dimers via an extensive interface, that is disrupted in receptor- and antibody-bound crystal structures, and, we propose a role for the dimer in virus stability and infectivity.
About this Structure
2GHW is a Single protein structure of sequence from Homo sapiens and Human sars coronavirus with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:16954221
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