2gk7
From Proteopedia
(New page: 200px<br /> <applet load="2gk7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gk7, resolution 2.80Å" /> '''Structural and Func...) |
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caption="2gk7, resolution 2.80Å" /> | caption="2gk7, resolution 2.80Å" /> | ||
'''Structural and Functional insights into the human Upf1 helicase core'''<br /> | '''Structural and Functional insights into the human Upf1 helicase core'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GK7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: upf1]] | [[Category: upf1]] | ||
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Revision as of 15:28, 15 February 2008
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Structural and Functional insights into the human Upf1 helicase core
Overview
Nonsense-mediated mRNA decay (NMD) is an mRNA surveillance pathway that, recognizes and degrades aberrant mRNAs containing premature stop codons. A, critical protein in NMD is Upf1p, which belongs to the helicase super, family 1 (SF1), and is thought to utilize the energy of ATP hydrolysis to, promote transitions in the structure of RNA or RNA-protein complexes. The, crystal structure of the catalytic core of human Upf1p determined in three, states (phosphate-, AMPPNP- and ADP-bound forms) reveals an overall, structure containing two RecA-like domains with two additional domains, protruding from the N-terminal RecA-like domain. Structural comparison, combined with mutational analysis identifies a likely single-stranded RNA, (ssRNA)-binding channel, and a cycle of conformational change coupled to, ATP binding and hydrolysis. These conformational changes alter the likely, ssRNA-binding channel in a manner that can explain how ATP binding, destabilizes ssRNA binding to Upf1p.
About this Structure
2GK7 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural and functional insights into the human Upf1 helicase core., Cheng Z, Muhlrad D, Lim MK, Parker R, Song H, EMBO J. 2007 Jan 10;26(1):253-64. Epub 2006 Dec 7. PMID:17159905
Page seeded by OCA on Fri Feb 15 17:28:18 2008
Categories: Homo sapiens | Single protein | Cheng, Z. | Muhlrad, D. | Parker, R. | Song, H. | PO4 | Helicase | Nmd | Upf1
