2i99
From Proteopedia
(New page: 200px<br /> <applet load="2i99" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i99, resolution 2.600Å" /> '''Crystal structure ...) |
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caption="2i99, resolution 2.600Å" /> | caption="2i99, resolution 2.600Å" /> | ||
'''Crystal structure of human Mu_crystallin at 2.6 Angstrom'''<br /> | '''Crystal structure of human Mu_crystallin at 2.6 Angstrom'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2I99 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NDP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2I99 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I99 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thyroid hormine binding protein]] | [[Category: thyroid hormine binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:35:18 2008'' |
Revision as of 15:35, 15 February 2008
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Crystal structure of human Mu_crystallin at 2.6 Angstrom
Contents |
Overview
Human cytosolic 3,5,3'-triiodo-L-thyronine-binding protein, also called, mu-crystallin or CRYM, plays important physiological roles in transporting, 3,5,3'-triiodo-L-thyronine (T(3)) into nuclei and regulating, thyroid-hormone-related gene expression. The crystal structure of human, CRYM's bacterial homolog Pseudomonas putida ornithine cyclodeaminase and, Archaeoglobus fulgidus alanine dehydrogenase have been available, but no, CRYM structure has been reported. Here, we report the crystal structure of, human CRYM bound with NADPH refined to 2.6 A, and there is one dimer in, the asymmetric unit. The structure contains two domains: a Rossmann, fold-like NADPH-binding domain and a dimerization domain. Different, conformations of the loop Arg83-His92 have been observed in two monomers, of human CRYM in the same asymmetric unit. The peptide bond of Val89-Pro90, is a trans-configuration in one monomer but a cis-configuration in the, other. A detailed comparison of the human mu-crystallin structure with its, structurally characterized homologs including the overall comparison and, superposition of active sites was conducted. Finally, a putative, T(3)-binding site in human CRYM is proposed based on comparison with, structural homologs.
Disease
Known disease associated with this structure: Deafness, autosomal dominant 40 OMIM:[123740]
About this Structure
2I99 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of human micro-crystallin complexed with NADPH., Cheng Z, Sun L, He J, Gong W, Protein Sci. 2007 Feb;16(2):329-35. PMID:17242435
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