2j4o
From Proteopedia
(New page: 200px<br /> <applet load="2j4o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j4o, resolution 2.25Å" /> '''STRUCTURE OF TAB1''...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2j4o. | + | [[Image:2j4o.jpg|left|200px]]<br /><applet load="2j4o" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j4o" size=" | + | |
caption="2j4o, resolution 2.25Å" /> | caption="2j4o, resolution 2.25Å" /> | ||
'''STRUCTURE OF TAB1'''<br /> | '''STRUCTURE OF TAB1'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2J4O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2J4O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4O OCA]. |
==Reference== | ==Reference== | ||
| Line 20: | Line 19: | ||
[[Category: tgf-beta]] | [[Category: tgf-beta]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:37:06 2008'' |
Revision as of 15:37, 15 February 2008
|
STRUCTURE OF TAB1
Overview
TAB1 [TAK1 (transforming growth factor-beta-activated kinase 1)-binding, protein 1] is one of the regulatory subunits of TAK1, a protein kinase, that lies at the head of three pro-inflammatory kinase cascades. In the, current study we report the crystal structure of the N-terminal domain of, TAB1. Surprisingly, TAB1 possesses a fold closely related to that of the, PPM (Mg2+- or Mn2+-dependent protein phosphatase) family as demonstrated, by the close structural similarity with protein phosphatase 2C alpha., However, we were unable to detect any phosphatase activity for TAB1 using, a phosphopeptide or p-nitrophenyl phosphate as substrate. Although the, overall protein phosphatase 2C alpha fold is conserved in TAB1, detailed, structural analyses and mutagenesis studies show that several key residues, required for dual metal-binding and catalysis are not present in TAB1, although binding of a single metal is supported by soaking experiments, with manganese and isothermal titration calorimetry. Thus, it appears that, TAB1 is a 'pseudophosphatase', possibly binding to and regulating, accessibility of phosphorylated residues on substrates downstream of TAK1, or on the TAK1 complex itself.
About this Structure
2J4O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
TAK1-binding protein 1 is a pseudophosphatase., Conner SH, Kular G, Peggie M, Shepherd S, Schuttelkopf AW, Cohen P, Van Aalten DM, Biochem J. 2006 Nov 1;399(3):427-34. PMID:16879102
Page seeded by OCA on Fri Feb 15 17:37:06 2008
