1grv
From Proteopedia
(New page: 200px<br /> <applet load="1grv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1grv, resolution 2.9Å" /> '''HYPOXANTHINE PHOSPHO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GRV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GRV OCA]]. | + | 1GRV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8]]. Structure known Active Site: MGA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GRV OCA]]. |
==Reference== | ==Reference== | ||
Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase., Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J, Protein Sci. 2002 Jul;11(7):1626-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12070315 12070315] | Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase., Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J, Protein Sci. 2002 Jul;11(7):1626-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12070315 12070315] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Hypoxanthine phosphoribosyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Guddat, L.W.]] | [[Category: Guddat, L.W.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:26:19 2007'' |
Revision as of 11:21, 30 October 2007
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HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE FROM E. COLI
Overview
Crystal structures have been determined for free Escherichia coli, hypoxanthine phosphoribosyltransferase (HPRT) (2.9 A resolution) and for, the enzyme in complex with the reaction products, inosine 5'-monophosphate, (IMP) and guanosine 5'-monophosphate (GMP) (2.8 A resolution). Of the, known 6-oxopurine phosphoribosyltransferase (PRTase) structures, E. coli, HPRT is most similar in structure to that of Tritrichomonas foetus HGXPRT, with a rmsd for 150 Calpha atoms of 1.0 A. Comparison of the free and, product bound structures shows that the side chain of Phe156 and the, polypeptide backbone in this vicinity move to bind IMP or GMP. A, nonproline cis peptide bond, also found in some other 6-oxopurine PRTases, is observed between Leu46 and Arg47 in both the free and complexed, structures. ... [(full description)]
About this Structure
1GRV is a [Single protein] structure of sequence from [Escherichia coli] with MG as [ligand]. Active as [Hypoxanthine phosphoribosyltransferase], with EC number [2.4.2.8]. Structure known Active Site: MGA. Full crystallographic information is available from [OCA].
Reference
Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase., Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J, Protein Sci. 2002 Jul;11(7):1626-38. PMID:12070315
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