397d
From Proteopedia
(New page: 200px<br /> <applet load="397d" size="450" color="white" frame="true" align="right" spinBox="true" caption="397d, resolution 1.300Å" /> '''A 1.3 A RESOLUTION...) |
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| - | [[Image:397d. | + | [[Image:397d.jpg|left|200px]]<br /><applet load="397d" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="397d, resolution 1.300Å" /> | caption="397d, resolution 1.300Å" /> | ||
'''A 1.3 A RESOLUTION CRYSTAL STRUCTURE OF THE HIV-1 TRANS-ACTIVATION RESPONSE REGION RNA STEM REVEALS A METAL ION-DEPENDENT BULGE CONFORMATION'''<br /> | '''A 1.3 A RESOLUTION CRYSTAL STRUCTURE OF THE HIV-1 TRANS-ACTIVATION RESPONSE REGION RNA STEM REVEALS A METAL ION-DEPENDENT BULGE CONFORMATION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 397D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 397D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=397D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: overhanging bases]] | [[Category: overhanging bases]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:42:50 2008'' |
Revision as of 15:42, 15 February 2008
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A 1.3 A RESOLUTION CRYSTAL STRUCTURE OF THE HIV-1 TRANS-ACTIVATION RESPONSE REGION RNA STEM REVEALS A METAL ION-DEPENDENT BULGE CONFORMATION
Overview
The crystal structure of an HIV-1 trans-activation response region (TAR), RNA fragment containing the binding site for the trans-activation protein, Tat has been determined to 1.3-A resolution. In this crystal structure, the characteristic UCU bulge of TAR adopts a conformation that is, stabilized by three divalent calcium ions and differs from those, determined previously by solution NMR. One metal ion, crucial to the loop, conformation, binds directly to three phosphates in the loop region. The, structure emphasizes the influence of metal ion binding on RNA structure, and, given the abundance of divalent metal ion in the cell, raises the, question of whether metal ions play a role in the conformation of TAR RNA, and the interaction of TAR with Tat and cyclin T in vivo.
About this Structure
397D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
A 1.3-A resolution crystal structure of the HIV-1 trans-activation response region RNA stem reveals a metal ion-dependent bulge conformation., Ippolito JA, Steitz TA, Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9819-24. PMID:9707559
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