1gvk
From Proteopedia
(New page: 200px<br /> <applet load="1gvk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gvk, resolution 0.945Å" /> '''PORCINE PANCREATIC...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GVK is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA, SO4 and ACE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVK OCA]]. | + | 1GVK is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA, SO4 and ACE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36]]. Structure known Active Site: CA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVK OCA]]. |
==Reference== | ==Reference== | ||
X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11896054 11896054] | X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11896054 11896054] | ||
| + | [[Category: Pancreatic elastase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:27:49 2007'' |
Revision as of 11:23, 30 October 2007
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PORCINE PANCREATIC ELASTASE ACYL ENZYME AT 0.95 A RESOLUTION
Overview
Kinetic analyses led to the discovery that N-acetylated tripeptides with, polar residues at P3 are inhibitors of porcine pancreatic elastase (PPE), that form unusually stable acyl-enzyme complexes. Peptides terminating in, a C-terminal carboxylate were more potent than those terminating in a, C-terminal amide, suggesting recognition by the oxy-anion hole is, important in binding. X-ray diffraction data were recorded to 0.95-A, resolution for an acyl-enzyme complex formed between PPE and, N-acetyl-Asn-Pro-Ile-CO2H at approximately pH 5. The accuracy of the, crystallographic coordinates allows structural issues concerning the, mechanism of serine proteases to be addressed. Significantly, the ester, bond of the acyl-enzyme showed a high level of planarity, suggesting, geometric strain of the ... [(full description)]
About this Structure
1GVK is a [Protein complex] structure of sequences from [Sus scrofa] with CA, SO4 and ACE as [ligands]. Active as [Pancreatic elastase], with EC number [3.4.21.36]. Structure known Active Site: CA. Full crystallographic information is available from [OCA].
Reference
X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:11896054
Page seeded by OCA on Tue Oct 30 13:27:49 2007
