1gvg
From Proteopedia
(New page: 200px<br /> <applet load="1gvg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gvg, resolution 1.54Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GVG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE, SO4, NO, AKG and PCX as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVG OCA]]. | + | 1GVG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]] with FE, SO4, NO, AKG and PCX as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVG OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: trifunctional enzyme]] | [[Category: trifunctional enzyme]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:28:10 2007'' |
Revision as of 11:23, 30 October 2007
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CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE
Overview
Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of, CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were, exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to, exposure with NO, the active site Fe(II) is octahedrally coordinated by a, water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the, side-chains of an aspartyl and two histidinyl residues. NO binds to the, position previously occupied by the 2OG 1-carboxylate concomitant with, rearrangement of the latter to the position previously occupied by the, displaced water.
About this Structure
1GVG is a [Single protein] structure of sequence from [Streptomyces clavuligerus] with FE, SO4, NO, AKG and PCX as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements., Zhang Z, Ren J, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ, FEBS Lett. 2002 Apr 24;517(1-3):7-12. PMID:12062399
Page seeded by OCA on Tue Oct 30 13:28:10 2007
Categories: Single protein | Streptomyces clavuligerus | Clifton, I.J. | Harlos, K. | Mckinnon, C.H. | Ren, J. | Schofield, C.J. | Zhang, Z.H. | AKG | FE | NO | PCX | SO4 | Clavaminate synthase 1 | Jelly roll | Lyase | Nitric oxide | Oxidoreductase | Oxygenase | Trifunctional enzyme
