153d

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(New page: 200px<br /><applet load="153d" size="450" color="white" frame="true" align="right" spinBox="true" caption="153d, resolution 2.900&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:153d.gif|left|200px]]<br /><applet load="153d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="153d, resolution 2.900&Aring;" />
caption="153d, resolution 2.900&Aring;" />
'''CRYSTAL STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O6ME)GCG)2, CONTAINING A CARCINOGENIC O6-METHYLGUANINE'''<br />
'''CRYSTAL STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O6ME)GCG)2, CONTAINING A CARCINOGENIC O6-METHYLGUANINE'''<br />
==Overview==
==Overview==
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The crystal structure of the synthetic deoxydodecamer, d(CGAGAATTC(O6Me)GCG)2 has been determined and refined to an R-factor of, 16.9% with data up to 2.9-A resolution. This sequence contains two, mismatched base pairs between O6-methylguanine and adenine with the, arrangement A(syn).(O6-Me)G(anti) which differs from the geometry observed, in solution by NMR. The intermolecular arrangement is equivalent to the, other isomorphous deoxydodecamers. However, the weakening of some, significant crystal packing contacts was observed and related to the, effect of stacking between the mispaired adenine and the adjacent guanine, in the sequence. The structure is highly hydrated, with a total of 49, solvent molecules located. The methyl group and the mismatched base-pair, geometry locally disrupt the B-DNA-type solvent network with two solvent, molecules found close to the N1 and N6 of the mispaired adenine.
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The crystal structure of the synthetic deoxydodecamer d(CGAGAATTC(O6Me)GCG)2 has been determined and refined to an R-factor of 16.9% with data up to 2.9-A resolution. This sequence contains two mismatched base pairs between O6-methylguanine and adenine with the arrangement A(syn).(O6-Me)G(anti) which differs from the geometry observed in solution by NMR. The intermolecular arrangement is equivalent to the other isomorphous deoxydodecamers. However, the weakening of some significant crystal packing contacts was observed and related to the effect of stacking between the mispaired adenine and the adjacent guanine in the sequence. The structure is highly hydrated, with a total of 49 solvent molecules located. The methyl group and the mismatched base-pair geometry locally disrupt the B-DNA-type solvent network with two solvent molecules found close to the N1 and N6 of the mispaired adenine.
==About this Structure==
==About this Structure==
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153D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=153D OCA].
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153D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=153D OCA].
==Reference==
==Reference==
Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine., Ginell SL, Vojtechovsky J, Gaffney B, Jones R, Berman HM, Biochemistry. 1994 Mar 29;33(12):3487-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8142345 8142345]
Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine., Ginell SL, Vojtechovsky J, Gaffney B, Jones R, Berman HM, Biochemistry. 1994 Mar 29;33(12):3487-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8142345 8142345]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Berman, H.M.]]
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[[Category: Berman, H M.]]
[[Category: Gaffney, B.]]
[[Category: Gaffney, B.]]
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[[Category: Ginell, S.L.]]
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[[Category: Ginell, S L.]]
[[Category: Jones, R.]]
[[Category: Jones, R.]]
[[Category: Vojtechovsky, J.]]
[[Category: Vojtechovsky, J.]]
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[[Category: modified]]
[[Category: modified]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:58:44 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:38:24 2008''

Revision as of 09:38, 21 February 2008

Image:153d.gif

153d, resolution 2.900Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF A MISPAIRED DODECAMER, D(CGAGAATTC(O6ME)GCG)2, CONTAINING A CARCINOGENIC O6-METHYLGUANINE

Overview

The crystal structure of the synthetic deoxydodecamer d(CGAGAATTC(O6Me)GCG)2 has been determined and refined to an R-factor of 16.9% with data up to 2.9-A resolution. This sequence contains two mismatched base pairs between O6-methylguanine and adenine with the arrangement A(syn).(O6-Me)G(anti) which differs from the geometry observed in solution by NMR. The intermolecular arrangement is equivalent to the other isomorphous deoxydodecamers. However, the weakening of some significant crystal packing contacts was observed and related to the effect of stacking between the mispaired adenine and the adjacent guanine in the sequence. The structure is highly hydrated, with a total of 49 solvent molecules located. The methyl group and the mismatched base-pair geometry locally disrupt the B-DNA-type solvent network with two solvent molecules found close to the N1 and N6 of the mispaired adenine.

About this Structure

153D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine., Ginell SL, Vojtechovsky J, Gaffney B, Jones R, Berman HM, Biochemistry. 1994 Mar 29;33(12):3487-93. PMID:8142345

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