178d

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(New page: 200px<br /><applet load="178d" size="450" color="white" frame="true" align="right" spinBox="true" caption="178d, resolution 2.500&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:178d.gif|left|200px]]<br /><applet load="178d" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:178d.gif|left|200px]]<br /><applet load="178d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="178d, resolution 2.500&Aring;" />
caption="178d, resolution 2.500&Aring;" />
'''CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS'''<br />
'''CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS'''<br />
==Overview==
==Overview==
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The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing, the chemically modified base 8-hydroxydeoxyguanine (O8G), has been, determined at 2.5-A resolution and refined to a crystallographic R-factor, of 16.8%. The B-type DNA helix contains standard Watson-Crick base pairs, except at the mismatch sites, where O8G adopts a syn conformation and, forms hydrogen bonds to adenine in the anti conformation. The, thermodynamic stability of the duplex was found by UV melting techniques, to be intermediate between the native oligonucleotide d(CGCAAATTTGCG) and, an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG). Comparison of, the structure of the O8G(syn).A(anti) base pair with those of Watson-Crick, base pairs has given a reason why O8G.A base pairs are not well repaired, by DNA proofreading enzymes.
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The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing the chemically modified base 8-hydroxydeoxyguanine (O8G), has been determined at 2.5-A resolution and refined to a crystallographic R-factor of 16.8%. The B-type DNA helix contains standard Watson-Crick base pairs except at the mismatch sites, where O8G adopts a syn conformation and forms hydrogen bonds to adenine in the anti conformation. The thermodynamic stability of the duplex was found by UV melting techniques to be intermediate between the native oligonucleotide d(CGCAAATTTGCG) and an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG). Comparison of the structure of the O8G(syn).A(anti) base pair with those of Watson-Crick base pairs has given a reason why O8G.A base pairs are not well repaired by DNA proofreading enzymes.
==About this Structure==
==About this Structure==
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178D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=178D OCA].
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178D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=178D OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Brown, T.]]
[[Category: Brown, T.]]
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[[Category: Gibson, N.J.]]
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[[Category: Gibson, N J.]]
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[[Category: Hunter, W.N.]]
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[[Category: Hunter, W N.]]
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[[Category: Leonard, G.A.]]
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[[Category: Leonard, G A.]]
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[[Category: McAuley-Hecht, K.E.]]
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[[Category: McAuley-Hecht, K E.]]
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[[Category: Thomson, J.B.]]
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[[Category: Thomson, J B.]]
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[[Category: Watson, W.P.]]
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[[Category: Watson, W P.]]
[[Category: b-dna]]
[[Category: b-dna]]
[[Category: double helix]]
[[Category: double helix]]
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[[Category: modified]]
[[Category: modified]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:06:30 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:38:52 2008''

Revision as of 09:38, 21 February 2008

Image:178d.gif

178d, resolution 2.500Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS

Overview

The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing the chemically modified base 8-hydroxydeoxyguanine (O8G), has been determined at 2.5-A resolution and refined to a crystallographic R-factor of 16.8%. The B-type DNA helix contains standard Watson-Crick base pairs except at the mismatch sites, where O8G adopts a syn conformation and forms hydrogen bonds to adenine in the anti conformation. The thermodynamic stability of the duplex was found by UV melting techniques to be intermediate between the native oligonucleotide d(CGCAAATTTGCG) and an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG). Comparison of the structure of the O8G(syn).A(anti) base pair with those of Watson-Crick base pairs has given a reason why O8G.A base pairs are not well repaired by DNA proofreading enzymes.

About this Structure

178D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Crystal structure of a DNA duplex containing 8-hydroxydeoxyguanine-adenine base pairs., McAuley-Hecht KE, Leonard GA, Gibson NJ, Thomson JB, Watson WP, Hunter WN, Brown T, Biochemistry. 1994 Aug 30;33(34):10266-70. PMID:8068665

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