183d
From Proteopedia
(New page: 200px<br /><applet load="183d" size="450" color="white" frame="true" align="right" spinBox="true" caption="183d, resolution 1.600Å" /> '''X-RAY STRUCTURE OF ...) |
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| - | [[Image:183d.gif|left|200px]]<br /><applet load="183d" size=" | + | [[Image:183d.gif|left|200px]]<br /><applet load="183d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="183d, resolution 1.600Å" /> | caption="183d, resolution 1.600Å" /> | ||
'''X-RAY STRUCTURE OF A DNA DECAMER CONTAINING 7, 8-DIHYDRO-8-OXOGUANINE'''<br /> | '''X-RAY STRUCTURE OF A DNA DECAMER CONTAINING 7, 8-DIHYDRO-8-OXOGUANINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the x-ray structure of a DNA fragment containing | + | We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The results demonstrate that GO forms Watson-Crick base pairs with the opposite C and that G(O) is in the anti conformation. Structural perturbations induced by C.G(O)anti base pairs are subtle. The structure allows us to identify probable elements by which the DNA repair protein MutM recognizes its substrates. Hydrogen bond donors/acceptors within the major groove are the most likely element. In that groove, the pattern of hydrogen-bond donors/acceptors of C.G(O)anti is unique. Additional structural analysis indicates that conversion of G to G(O) would not significantly influence the glycosidic torsion preference of the nucleoside. There is no steric interaction of the 8-oxygen of G(O) with the phospho-deoxyribose backbone. |
==About this Structure== | ==About this Structure== | ||
| - | 183D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 183D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=183D OCA]. |
==Reference== | ==Reference== | ||
X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine., Lipscomb LA, Peek ME, Morningstar ML, Verghis SM, Miller EM, Rich A, Essigmann JM, Williams LD, Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):719-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7846041 7846041] | X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine., Lipscomb LA, Peek ME, Morningstar ML, Verghis SM, Miller EM, Rich A, Essigmann JM, Williams LD, Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):719-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7846041 7846041] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Essigmann, J | + | [[Category: Essigmann, J M.]] |
| - | [[Category: Lipscomb, L | + | [[Category: Lipscomb, L A.]] |
| - | [[Category: Miller, E | + | [[Category: Miller, E M.]] |
| - | [[Category: Morningstar, M | + | [[Category: Morningstar, M L.]] |
| - | [[Category: Peek, M | + | [[Category: Peek, M E.]] |
[[Category: Rich, A.]] | [[Category: Rich, A.]] | ||
| - | [[Category: Verghis, S | + | [[Category: Verghis, S M.]] |
| - | [[Category: Williams, L | + | [[Category: Williams, L D.]] |
[[Category: b-dna]] | [[Category: b-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: modified]] | [[Category: modified]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:38:58 2008'' |
Revision as of 09:39, 21 February 2008
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X-RAY STRUCTURE OF A DNA DECAMER CONTAINING 7, 8-DIHYDRO-8-OXOGUANINE
Overview
We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The results demonstrate that GO forms Watson-Crick base pairs with the opposite C and that G(O) is in the anti conformation. Structural perturbations induced by C.G(O)anti base pairs are subtle. The structure allows us to identify probable elements by which the DNA repair protein MutM recognizes its substrates. Hydrogen bond donors/acceptors within the major groove are the most likely element. In that groove, the pattern of hydrogen-bond donors/acceptors of C.G(O)anti is unique. Additional structural analysis indicates that conversion of G to G(O) would not significantly influence the glycosidic torsion preference of the nucleoside. There is no steric interaction of the 8-oxygen of G(O) with the phospho-deoxyribose backbone.
About this Structure
183D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine., Lipscomb LA, Peek ME, Morningstar ML, Verghis SM, Miller EM, Rich A, Essigmann JM, Williams LD, Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):719-23. PMID:7846041
Page seeded by OCA on Thu Feb 21 11:38:58 2008
