1a5q
From Proteopedia
(New page: 200px<br /><applet load="1a5q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a5q, resolution 2.3Å" /> '''P93A VARIANT OF BOVIN...) |
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| - | [[Image:1a5q.gif|left|200px]]<br /><applet load="1a5q" size=" | + | [[Image:1a5q.gif|left|200px]]<br /><applet load="1a5q" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1a5q, resolution 2.3Å" /> | caption="1a5q, resolution 2.3Å" /> | ||
'''P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A'''<br /> | '''P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase | + | The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the Tyr92-Ala93 peptide group also exists in the cis conformation in the native state. Here, we report the crystal structure of the P93A variant. Although there is disorder in the region of residues 92 and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report the crystal structure of the C[40, 95]A variant, which is an analog of the major rate-determining three-disulfide intermediate in the oxidative folding of RNase A, missing the 40-95 disulfide bond. As had been detected by NMR spectroscopy, the crystal structure of this analog shows disorder in the region surrounding the missing disulfide. However, the global chain fold of the remainder of the protein, including the disulfide bond between Cys65 and Cys72, appears to be unaffected by the mutation. |
==About this Structure== | ==About this Structure== | ||
| - | 1A5Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http:// | + | 1A5Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5Q OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dodge, R | + | [[Category: Dodge, R W.]] |
| - | [[Category: Karplus, P | + | [[Category: Karplus, P A.]] |
| - | [[Category: Laity, J | + | [[Category: Laity, J H.]] |
| - | [[Category: Pearson, M | + | [[Category: Pearson, M A.]] |
| - | [[Category: Scheraga, H | + | [[Category: Scheraga, H A.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: hydrolase (nucleic acid]] | [[Category: hydrolase (nucleic acid]] | ||
[[Category: rna)]] | [[Category: rna)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:09 2008'' |
Revision as of 09:41, 21 February 2008
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P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A
Overview
The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the Tyr92-Ala93 peptide group also exists in the cis conformation in the native state. Here, we report the crystal structure of the P93A variant. Although there is disorder in the region of residues 92 and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report the crystal structure of the C[40, 95]A variant, which is an analog of the major rate-determining three-disulfide intermediate in the oxidative folding of RNase A, missing the 40-95 disulfide bond. As had been detected by NMR spectroscopy, the crystal structure of this analog shows disorder in the region surrounding the missing disulfide. However, the global chain fold of the remainder of the protein, including the disulfide bond between Cys65 and Cys72, appears to be unaffected by the mutation.
About this Structure
1A5Q is a Single protein structure of sequence from Bos taurus with as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
Crystal structures of two mutants that have implications for the folding of bovine pancreatic ribonuclease A., Pearson MA, Karplus PA, Dodge RW, Laity JH, Scheraga HA, Protein Sci. 1998 May;7(5):1255-8. PMID:9605332
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