1a60
From Proteopedia
(New page: 200px<br /><applet load="1a60" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a60" /> '''NMR STRUCTURE OF A CLASSICAL PSEUDOKNOT: INT...) |
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'''NMR STRUCTURE OF A CLASSICAL PSEUDOKNOT: INTERPLAY OF SINGLE-AND DOUBLE-STRANDED RNA, 24 STRUCTURES'''<br /> | '''NMR STRUCTURE OF A CLASSICAL PSEUDOKNOT: INTERPLAY OF SINGLE-AND DOUBLE-STRANDED RNA, 24 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pseudoknot formation folds the 3' ends of many plant viral genomic RNAs | + | Pseudoknot formation folds the 3' ends of many plant viral genomic RNAs into structures that resemble transfer RNA in global folding and in their reactivity to transfer RNA-specific proteins. The solution structure of the pseudoknotted T arm and acceptor arm of the transfer RNA-like structure of turnip yellow mosaic virus (TYMV) was determined by nuclear magnetic resonance (NMR) spectroscopy. The molecule is stabilized by the hairpin formed by the 5' end of the RNA, and by the intricate interactions related to the loops of the pseudoknot. Loop 1 spans the major groove of the helix with only two of its four nucleotides. Loop 2, which crosses the minor groove, interacts closely with its opposing helix, in particular through hydrogen bonds with a highly conserved adenine. The structure resulting from this interaction between the minor groove and single-stranded RNA at helical junctions displays internal mobility, which may be a general feature of RNA pseudoknots that regulates their interaction with proteins or other RNA molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1A60 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Turnip_yellow_mosaic_virus Turnip yellow mosaic virus]. Full crystallographic information is available from [http:// | + | 1A60 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Turnip_yellow_mosaic_virus Turnip yellow mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A60 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Turnip yellow mosaic virus]] | [[Category: Turnip yellow mosaic virus]] | ||
| - | [[Category: Graaf, M | + | [[Category: Graaf, M Van Der.]] |
| - | [[Category: Heus, H | + | [[Category: Heus, H A.]] |
| - | [[Category: Hilbers, C | + | [[Category: Hilbers, C W.]] |
| - | [[Category: Kolk, M | + | [[Category: Kolk, M H.]] |
| - | [[Category: Pleij, C | + | [[Category: Pleij, C W.A.]] |
| - | [[Category: Wijmenga, S | + | [[Category: Wijmenga, S S.]] |
[[Category: pseudoknot]] | [[Category: pseudoknot]] | ||
[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| Line 24: | Line 24: | ||
[[Category: tymv]] | [[Category: tymv]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:13 2008'' |
Revision as of 09:41, 21 February 2008
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NMR STRUCTURE OF A CLASSICAL PSEUDOKNOT: INTERPLAY OF SINGLE-AND DOUBLE-STRANDED RNA, 24 STRUCTURES
Overview
Pseudoknot formation folds the 3' ends of many plant viral genomic RNAs into structures that resemble transfer RNA in global folding and in their reactivity to transfer RNA-specific proteins. The solution structure of the pseudoknotted T arm and acceptor arm of the transfer RNA-like structure of turnip yellow mosaic virus (TYMV) was determined by nuclear magnetic resonance (NMR) spectroscopy. The molecule is stabilized by the hairpin formed by the 5' end of the RNA, and by the intricate interactions related to the loops of the pseudoknot. Loop 1 spans the major groove of the helix with only two of its four nucleotides. Loop 2, which crosses the minor groove, interacts closely with its opposing helix, in particular through hydrogen bonds with a highly conserved adenine. The structure resulting from this interaction between the minor groove and single-stranded RNA at helical junctions displays internal mobility, which may be a general feature of RNA pseudoknots that regulates their interaction with proteins or other RNA molecules.
About this Structure
1A60 is a Protein complex structure of sequences from Turnip yellow mosaic virus. Full crystallographic information is available from OCA.
Reference
NMR structure of a classical pseudoknot: interplay of single- and double-stranded RNA., Kolk MH, van der Graaf M, Wijmenga SS, Pleij CW, Heus HA, Hilbers CW, Science. 1998 Apr 17;280(5362):434-8. PMID:9545221
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