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1a77
From Proteopedia
(New page: 200px<br /><applet load="1a77" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a77, resolution 2.00Å" /> '''FLAP ENDONUCLEASE-1 ...) |
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| - | [[Image:1a77.jpg|left|200px]]<br /><applet load="1a77" size=" | + | [[Image:1a77.jpg|left|200px]]<br /><applet load="1a77" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1a77, resolution 2.00Å" /> | caption="1a77, resolution 2.00Å" /> | ||
'''FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII'''<br /> | '''FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an | + | Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1A77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1A77 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A77 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Baek, K.]] | [[Category: Baek, K.]] | ||
[[Category: Cho, Y.]] | [[Category: Cho, Y.]] | ||
| - | [[Category: Hwang, K | + | [[Category: Hwang, K Y.]] |
[[Category: Kim, H.]] | [[Category: Kim, H.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 24: | Line 24: | ||
[[Category: rth]] | [[Category: rth]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:40 2008'' |
Revision as of 09:41, 21 February 2008
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FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
Overview
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
About this Structure
1A77 is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:9699635
Page seeded by OCA on Thu Feb 21 11:41:40 2008
