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1aac
From Proteopedia
(New page: 200px<br /><applet load="1aac" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aac, resolution 1.31Å" /> '''AMICYANIN OXIDIZED, ...) |
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| - | [[Image:1aac.gif|left|200px]]<br /><applet load="1aac" size=" | + | [[Image:1aac.gif|left|200px]]<br /><applet load="1aac" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1aac, resolution 1.31Å" /> | caption="1aac, resolution 1.31Å" /> | ||
'''AMICYANIN OXIDIZED, 1.31 ANGSTROMS'''<br /> | '''AMICYANIN OXIDIZED, 1.31 ANGSTROMS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | High-resolution X-ray diffraction data to d(min) = 1.31 A were collected | + | High-resolution X-ray diffraction data to d(min) = 1.31 A were collected on a Xuong-Hamlin area detector from crystals of the blue-copper protein amicyanin, isolated from P. denitrificans. With coordinates from the earlier 2.0 A structure determination as a starting point, simulated annealing and restrained positional and temperature factor refinements using the program X-PLOR resulted in a final R factor of 15.5%, based on 21 131 unique reflections in the range 8.0-1.3 A. Comparison of the 1.31 A structure with that at 2.0 A shows the same basic features. However, the high-resolution electron-density maps clearly reveal additional solvent molecules and significant discrete disorder in protein side chains and within the solvent structure. As a consequence of modelling side-chain disorder, several new hydrogen-bonding interactions were identified. |
==About this Structure== | ==About this Structure== | ||
| - | 1AAC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1AAC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AAC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Paracoccus denitrificans]] | [[Category: Paracoccus denitrificans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, Z | + | [[Category: Chen, Z W.]] |
| - | [[Category: Cunane, L | + | [[Category: Cunane, L M.]] |
| - | [[Category: Durley, R | + | [[Category: Durley, R C.E.]] |
| - | [[Category: Mathews, F | + | [[Category: Mathews, F S.]] |
[[Category: CU]] | [[Category: CU]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:36 2008'' |
Revision as of 09:42, 21 February 2008
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AMICYANIN OXIDIZED, 1.31 ANGSTROMS
Overview
High-resolution X-ray diffraction data to d(min) = 1.31 A were collected on a Xuong-Hamlin area detector from crystals of the blue-copper protein amicyanin, isolated from P. denitrificans. With coordinates from the earlier 2.0 A structure determination as a starting point, simulated annealing and restrained positional and temperature factor refinements using the program X-PLOR resulted in a final R factor of 15.5%, based on 21 131 unique reflections in the range 8.0-1.3 A. Comparison of the 1.31 A structure with that at 2.0 A shows the same basic features. However, the high-resolution electron-density maps clearly reveal additional solvent molecules and significant discrete disorder in protein side chains and within the solvent structure. As a consequence of modelling side-chain disorder, several new hydrogen-bonding interactions were identified.
About this Structure
1AAC is a Single protein structure of sequence from Paracoccus denitrificans with as ligand. Full crystallographic information is available from OCA.
Reference
X-ray structure of the cupredoxin amicyanin, from Paracoccus denitrificans, refined at 1.31 A resolution., Cunane LM, Chen ZW, Durley RC, Mathews FS, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):676-86. PMID:15299631
Page seeded by OCA on Thu Feb 21 11:42:36 2008
