1af5
From Proteopedia
(New page: 200px<br /><applet load="1af5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1af5, resolution 3.00Å" /> '''GROUP I MOBILE INTRO...) |
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| - | [[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size=" | + | [[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1af5, resolution 3.00Å" /> | caption="1af5, resolution 3.00Å" /> | ||
'''GROUP I MOBILE INTRON ENDONUCLEASE'''<br /> | '''GROUP I MOBILE INTRON ENDONUCLEASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of I-Crel provides the first view of a protein encoded by a | + | The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site. |
==About this Structure== | ==About this Structure== | ||
| - | 1AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http:// | + | 1AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Heath, P | + | [[Category: Heath, P J.]] |
| - | [[Category: Junior, R | + | [[Category: Junior, R J.Monnat.]] |
| - | [[Category: Stephens, K | + | [[Category: Stephens, K M.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: chloroplast dna]] | [[Category: chloroplast dna]] | ||
[[Category: endonuclease]] | [[Category: endonuclease]] | ||
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[[Category: laglidadg motif]] | [[Category: laglidadg motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:57 2008'' |
Revision as of 09:44, 21 February 2008
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GROUP I MOBILE INTRON ENDONUCLEASE
Overview
The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.
About this Structure
1AF5 is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.
Reference
The structure of I-Crel, a group I intron-encoded homing endonuclease., Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1997 Jun;4(6):468-76. PMID:9187655
Page seeded by OCA on Thu Feb 21 11:43:57 2008
