1afx
From Proteopedia
(New page: 200px<br /><applet load="1afx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1afx" /> '''UGAA EUKARYOTIC RIBOSOMAL RNA TETRALOOP, NMR...) |
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'''UGAA EUKARYOTIC RIBOSOMAL RNA TETRALOOP, NMR, 13 STRUCTURES'''<br /> | '''UGAA EUKARYOTIC RIBOSOMAL RNA TETRALOOP, NMR, 13 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The hairpin ribozyme is a small catalytic RNA with a unique two-domain | + | The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1AFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1AFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AFX OCA]. |
==Reference== | ==Reference== | ||
Solution structure of the loop B domain from the hairpin ribozyme., Butcher SE, Allain FH, Feigon J, Nat Struct Biol. 1999 Mar;6(3):212-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074938 10074938] | Solution structure of the loop B domain from the hairpin ribozyme., Butcher SE, Allain FH, Feigon J, Nat Struct Biol. 1999 Mar;6(3):212-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074938 10074938] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Butcher, S | + | [[Category: Butcher, S E.]] |
[[Category: Dieckmann, T.]] | [[Category: Dieckmann, T.]] | ||
[[Category: Feigon, J.]] | [[Category: Feigon, J.]] | ||
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[[Category: tetraloop]] | [[Category: tetraloop]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:05 2008'' |
Revision as of 09:44, 21 February 2008
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UGAA EUKARYOTIC RIBOSOMAL RNA TETRALOOP, NMR, 13 STRUCTURES
Overview
The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain.
About this Structure
1AFX is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of the loop B domain from the hairpin ribozyme., Butcher SE, Allain FH, Feigon J, Nat Struct Biol. 1999 Mar;6(3):212-6. PMID:10074938
Page seeded by OCA on Thu Feb 21 11:44:05 2008
